Coordinated assembly and release of adhesions builds apical junctional belts during de novo polarisation of an epithelial tube
| Autor: | Charlotte A. Williams, Jonathan D.W. Clarke, Andrew C. Symonds, Clare E. Buckley |
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| Přispěvatelé: | Buckley, Clare E [0000-0003-3329-3973], Clarke, Jonathan DW [0000-0003-0223-2860], Apollo - University of Cambridge Repository |
| Rok vydání: | 2020 |
| Předmět: |
Neural Tube
Adhesions Dual role medicine Morphogenesis Animals Zebrafish biology Chemistry Neural tube Cell Polarity Gene Expression Regulation Developmental Membrane Proteins Epithelial Cells Zebrafish Proteins biology.organism_classification Transmembrane protein Cell biology medicine.anatomical_structure Intercellular Junctions Guanylate Cyclase rab GTP-Binding Proteins Epithelial tube RAB11A Lumen (unit) |
| Popis: | Using the zebrafish neural tube as a model, we uncover thein vivomechanisms allowing the generation of two opposing apical epithelial surfaces within the centre of an initially unpolarised, solid organ. We show that NOK/Pals1/Mpp5a and Rab11a play a dual role in coordinating the generation of ipsilateral junctional belts whilst simultaneously releasing contralateral adhesions across the centre of the tissue. We show that Nok and Rab11a mediated resolution of cell-cell adhesions are both necessary for midline lumen opening and contribute to later maintenance of epithelial organisation. We propose these roles for both Nok and Rab11a operate through the transmembrane protein Crumbs. In light of a recent conflicting publication, we also clarify that the junction remodelling role of Nok is not specific to dividing cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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