MALDI imaging mass spectrometry reveals COX7A2, TAGLN2 and S100-A10 as novel prognostic markers in Barrett's adenocarcinoma
| Autor: | Hakan Sarioglu, Axel Walch, Sandra Rauser, Bernhard Kuster, Benjamin Balluff, Mareike Elsner, Gerhard Jung, Michaela Aichler, Cédrik Schöne, Heinz Höfler, Horst Zitzelsberger, Giuseppina Maccarrone, Stephan Meding, Marcus Feith, Uta Jütting, Stefan Maier, Marius Ueffing, Rupert Langer, Martin Nipp, Annette Feuchtinger |
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| Rok vydání: | 2012 |
| Předmět: |
Male
Proteomics MALDI imaging Biophysics Muscle Proteins Adenocarcinoma Biology Bioinformatics Mass spectrometry medicine.disease_cause Biochemistry Electron Transport Complex IV Barrett's Adenocarcinoma Biomarkers Tumor medicine Humans Neoplasm Invasiveness Annexin A2 Microfilament Proteins S100 Proteins Cancer Prognosis medicine.disease Immunohistochemistry digestive system diseases Gene Expression Regulation Neoplastic Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Cancer research Female Carcinogenesis |
| Zdroj: | Journal of Proteomics. 75:4693-4704 |
| ISSN: | 1874-3919 |
| Popis: | To characterize proteomic changes found in Barrett's adenocarcinoma and its premalignant stages, the proteomic profiles of histologically defined precursor and invasive carcinoma lesions were analyzed by MALDI imaging MS. For a primary proteomic screening, a discovery cohort of 38 fresh frozen Barrett's adenocarcinoma patient tissue samples was used. The goal was to find proteins that might be used as markers for monitoring cancer development as well as for predicting regional lymph node metastasis and disease outcome. Using mass spectrometry for protein identification and validating the results by immunohistochemistry on an independent validation set, we could identify two of 60 differentially expressed m/z species between Barrett's adenocarcinoma and the precursor lesion: COX7A2 and S100-A10. Furthermore, among 22 m/z species that are differentially expressed in Barrett's adenocarcinoma cases with and without regional lymph node metastasis, one was identified as TAGLN2. In the validation set, we found a correlation of the expression levels of COX7A2 and TAGLN2 with a poor prognosis while S100-A10 was confirmed by multivariate analysis as a novel independent prognostic factor in Barrett's adenocarcinoma. Our results underscore the high potential of MALDI imaging for revealing new biologically significant molecular details from cancer tissues which might have potential for clinical application. This article is part of a Special Issue entitled: Translational Proteomics. |
| Databáze: | OpenAIRE |
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