Dioleoyl phosphatidic acid induces morphological changes through an endogenous LPA receptor in C6 glioma cells
| Autor: | Hyo-Lim Kim, Yu-Lee Kim, Dong-Soon Im, Ji-Yeong Jo, Young-Ja Chang, Kim Kyeok |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
medicine.drug_class
G protein Cell Survival Pyridines Phosphatidic Acids Biology Cell morphology Pertussis toxin chemistry.chemical_compound Cell Line Tumor Drug Discovery Lysophosphatidic acid medicine Animals Receptors Lysophosphatidic Acid Receptor G protein-coupled receptor Cytotoxins Organic Chemistry Isoproterenol Phosphatidic acid Glioma Isoxazoles Receptor antagonist Organophosphates Cell biology Rats Drug Partial Agonism chemistry Pertussis Toxin Molecular Medicine lipids (amino acids peptides and proteins) biological phenomena cell phenomena and immunity Lysophospholipids Propionates |
| Zdroj: | Archives of pharmacal research. 31(5) |
| ISSN: | 0253-6269 |
| Popis: | Previously, we suggested that dioleoyl phosphatidic acid (PA) and lysophosphatidic acid (LPA) increased [Ca(2+)](i) through endogenous LPA receptors coupled to pertussis toxin-sensitive G proteins in rat C6 glioma cells. In the present report, we investigated morphological changes and cytotoxicity induced by PA and LPA in C6 glioma cells. Isoproterenol treatment led to changes in the cell morphology of rat C6 glioma cells, which were reverted by the addition of PA and LPA. PA-and LPA-induced morphological reversions were inhibited by treatment with Ki16425, an LPA(1)/LPA(3) receptor antagonist. VPC32183, another LPA(1)/LPA(3) receptor antagonist with a different structure, only inhibited PA-induced morphological reversion but not LPA-induced reversion. However, the reversions were not inhibited by treatment with pertussis toxin, a specific inhibitor of G(i/o) proteins. In addition, cytotoxicity was only induced by LPA but not by PA in C6 glioma cells. Our results suggest that PA may act as a partial agonist at endogenous LPA receptors, which are sensitive to Ki16425 and coupled to PTX-insensitive G proteins, to evoke morphological changes in C6 glioma cells. |
| Databáze: | OpenAIRE |
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