Evaluating the Intracellular Stability and Unpacking of DNA Nanocomplexes by Quantum Dots-FRET
| Autor: | Ho, Yi-Ping, Chen, Hunter H, Leong, Kam W, Wang, Tza-Huei, Ho, Megan Yi-Ping |
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| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
Glycoside Hydrolases
Pharmaceutical Science Nanotechnology Gene delivery Article chemistry.chemical_compound Microscopy Electron Transmission Cell Line Tumor Fluorescence microscope Fluorescence Resonance Energy Transfer Humans Particle Size Fluorescent Dyes Chitosan Microscopy Confocal Heparin Genetic transfer Rational design Gene Transfer Techniques DNA Fluorescence Förster resonance energy transfer Spectrometry Fluorescence chemistry Microscopy Fluorescence Quantum dot Nanoparticles Tumor Humans Chitosan Microscopy: Fluorescence Fluorescence Resonance Energy Transfer Spectrometry: Fluorescence Nanoparticles DNA Heparin Fluorescent Dyes Microscopy: Electron: Transmission Microscopy: Confocal Glycoside Hydrolases [Particle Size Gene Transfer Techniques Cell Line] |
| Zdroj: | Ho, Y-P, Chen, H H, Leong, K W, Wang, T-H & Ho, M Y-P 2006, ' Evaluating the intracellular stability and unpacking of DNA nanocomplexes by quantum dots-FRET ', Journal of controlled release : official journal of the Controlled Release Society, vol. 116, no. 1, pp. 83-9 . https://doi.org/10.1016/j.jconrel.2006.09.005 |
| DOI: | 10.1016/j.jconrel.2006.09.005 |
| Popis: | We demonstrate a highly sensitive method to characterize the structural composition and intracellular fate of polymeric DNA nanocomplexes, formed by condensing plasmid DNA with cationic polymers through electrostatic interactions. Rational design of more efficient polymeric gene carriers will be possible only with mechanistic insights of the rate-limiting steps in the non-viral gene transfer process. To characterize the composition and binding dynamics of nanocomplexes, plasmid and its polymer carrier within nanocomplexes were labeled with quantum dots (QDs) and fluorescent organic dyes, respectively, as a donor and acceptor pair for fluorescence resonance energy transfer (FRET). The high signal-to-noise ratio in QD-mediated FRET enabled precise detection of discrete changes in nanocomplex state at the single-particle level, against various intracellular microenvironments. The distribution and unpacking of individual nanocomplexes within cells could thus be unambiguously followed by fluorescence microscopy. QD-FRET is a highly sensitive and quantitative method to determine the composition and dynamic stability of nanocomplexes during intracellular transport, where barriers to gene delivery may be identified to facilitate gene carrier optimization. |
| Databáze: | OpenAIRE |
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