Preparation and characterization of PLLA/chitosan-graft-poly (ε-caprolactone) (CS-g-PCL) composite fibrous mats: The microstructure, performance and proliferation assessment
| Autor: | Liming Zou, Bo Liu, Li Wengang, Chenkai Sun, Yongjing Xu |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Absorption of water
Materials science Magnetic Resonance Spectroscopy Surface Properties Polyesters Composite number Nanofibers Biocompatible Materials 02 engineering and technology Biochemistry Cell Line Chitosan Contact angle 03 medical and health sciences chemistry.chemical_compound Mice X-Ray Diffraction Structural Biology Materials Testing Spectroscopy Fourier Transform Infrared Animals Molecular Biology 030304 developmental biology Cell Proliferation 0303 health sciences Temperature Water General Medicine Fibroblasts 021001 nanoscience & nanotechnology Electrospinning Solvent chemistry Chemical engineering Solubility Nanofiber 0210 nano-technology Caprolactone Porosity |
| Zdroj: | International journal of biological macromolecules. 162 |
| ISSN: | 1879-0003 |
| Popis: | In order to achieve the electrospinning of chitosan in common organic solvents, amino-reserved CS-g-PCL was synthesized by one-step method. PLLA and dichloromethane/ethanol (CH2Cl2/EtOH) solvent were chosen to prepare a series of different compositions of PLLA/CS-g-PCL mats (8/2, 6/4, 4/6, 2/8 wt/wt%) by electrospinning. The SEM showed that the smooth defect-free and uniform nanofibers were collected except PLLA/CS-g-PCL 2/8 and pure CS-g-PCL, and the fiber diameter was decreased from 828 nm to 461 nm with the increasing content of CS-g-PCL. The mechanical properties of composite mats have decreased with increasing CS-g-PCL content, but much higher than neat PLLA. The water contact angle, water absorption rate, water-vapor transmission rate and in vitro degradation behavior were 129°–19°, 109%–482%, 1945–2517 g m−2 day−1 and 4–14%, respectively. The addition of CS-g-PCL imparted PLLA/CS-g-PCL electrospun mats on better properties, improving the nature defects of PLLA. The in vitro cell culture studies showed that PLLA/CS-g-PCL 6/4 exhibited a higher in vitro biocompatibility and a better ability for cell attachment, spreading, and proliferation, comparing with PLLA mats. Herein, PLLA/CS-g-PCL 6/4 electrospun mats with excellent performance, was considered the potential application as wound dressing in skin tissue engineering. |
| Databáze: | OpenAIRE |
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