Applicability of an Ultrasonic Nebulization System for the Airways Delivery of Beclomethasone Dipropionate in a Murine Model of Asthma
| Autor: | Boska Hrvacic, Berislav Bošnjak, Milan Mesic, Marijan Tudja, Mladen Mercep |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Male
Ovalbumin Pharmaceutical Science Mice Respiratory Hypersensitivity medicine Animals Ultrasonics Pharmacology (medical) Anti-Asthmatic Agents Particle Size Aerosolization Pharmacology Mice Inbred BALB C Chromatography medicine.diagnostic_test Inhalation business.industry Nebulizers and Vaporizers Organic Chemistry Beclomethasone Beclometasone dipropionate respiratory system Asthma Aerosol Nebulizer Bronchoalveolar lavage asthma beclomethasone dipropionate dry powder inhalation delivery mice ultrasonic nebulization system Immunology Molecular Medicine Nasal administration Particle size Powders business Bronchoalveolar Lavage Fluid Algorithms Biotechnology medicine.drug |
| Zdroj: | Pharmaceutical Research. 23:1765-1775 |
| ISSN: | 1573-904X 0724-8741 |
| DOI: | 10.1007/s11095-006-9029-y |
| Popis: | Purpose. We have assessed the use of an ultrasonic nebulization system (UNS), composed of ultrasonic nebulizer and diffusion dryer filled with charcoal, for the effective delivery of beclomethasone to the airways in a murine asthma model. Methods. Solution of beclomethasone in ethanol was aerosolized using an ultrasonic nebulizer. Passage of the aerosol through a drying column containing charcoal and deionizer produced dry beclomethasone particles. Particles were delivered to BALB/c mice placed in a whole-body exposition chamber 1 h before intranasal challenge with ovalbumine. Efficacy of beclomethasone delivery was evaluated by examining bronchoalveolar lavage fluid (BALF) cytology. Results. Effect of three UNS system parameters on aerosol particle size was investigated. The critical parameter affecting the size of dry particles was beclomethasone concentration in aerosolized solution and solution flow rate while power level of ultrasonic nebulizer generator had no effect. Administration of beclomethasone at calculated dose of 150 mg/kg to mice significantly decreased total cell number and relative eosinophil number in BALF. Conclusions. The UNS system produces a monodisperse aerosol that can be used for inhalative delivery of poorly water soluble substances to experimental animals. The UNS system minimizes formulation requirements and allows rapid and relatively simple efficacy and toxicity testing in animals. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink