COP1 mediates light-dependent regulation of flavonol biosynthesis through HY5 in Arabidopsis
Autor: | Ashutosh Pandey, Subhash Reddy Gaddam, Prabodh Kumar Trivedi, Chitra Bhatia |
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Rok vydání: | 2021 |
Předmět: |
0106 biological sciences
0301 basic medicine Flavonols Light Ultraviolet Rays Ubiquitin-Protein Ligases Arabidopsis Plant Science Biology Real-Time Polymerase Chain Reaction 01 natural sciences Hypocotyl 03 medical and health sciences chemistry.chemical_compound Biosynthesis Gene Expression Regulation Plant Two-Hybrid System Techniques Genetics Gene Psychological repression chemistry.chemical_classification Arabidopsis Proteins fungi General Medicine Plants Genetically Modified biology.organism_classification Cell biology Ubiquitin ligase Basic-Leucine Zipper Transcription Factors 030104 developmental biology Flavonoid biosynthesis chemistry biology.protein Agronomy and Crop Science Transcription Factors 010606 plant biology & botany |
Zdroj: | Plant Science. 303:110760 |
ISSN: | 0168-9452 |
Popis: | Flavonols, a class of flavonoids, accumulate as protective agents in response to various stresses. Among various environmental stimuli, light is one of the factors regulating flavonol production. MYB12/11/111, members of the R2R3 MYBs family, regulates spatio-temporal flavonol accumulation in Arabidopsis. Although various studies indicate at the involvement of an E3 ubiquitin ligase CONSTITUTIVE PHOTOMORPHOGENIC 1 (COP1) and ELONGATED HYPOCOTYL 5 (HY5) in flavonoid biosynthesis in response to UV-B, the regulatory roles of these components under visible light are yet to be investigated. Here, we demonstrate that flavonol accumulation in Arabidopsis is light-regulated. Furthermore, our analysis suggests that MYB12 is a HY5-dependent light-inducible gene and plays a key role in the activation of the flavonol biosynthesis in response to light. Our results indicate the involvement of COP1 in the dark-dependent repression of MYB12 expression and flavonol accumulation. In addition, results also suggest that the effect of COP1 on MYB12 is indirect and is mediated through HY5, a direct transcriptional activator of the MYB12. Together these findings indicate that COP1 acts as a master negative regulator of flavonol biosynthesis in the dark. |
Databáze: | OpenAIRE |
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