The extracellular proteome of Bacillus licheniformis grown in different media and under different nutrient starvation conditions
| Autor: | Mark J. J. B. Sibbald, Birgit Voigt, Karl-Heinz Maurer, Armin Ehrenreich, Michael Hecker, Dirk Albrecht, Gerhard Gottschalk, Jan Maarten van Dijl, Jörg Feesche, Thomas Schweder, Jörg Bernhardt |
|---|---|
| Přispěvatelé: | Translational Immunology Groningen (TRIGR) |
| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
Signal peptide
0106 biological sciences Proteome Nitrogen Molecular Sequence Data Bacillus PHO Flagellum Proteomics SUBTILIS 01 natural sciences Biochemistry ELECTROPHORESIS Phosphates 03 medical and health sciences Bacterial Proteins nutrient starvation 010608 biotechnology REVEALS Extracellular Bacillus licheniformis Secretion Electrophoresis Gel Two-Dimensional Amino Acid Sequence Molecular Biology REGULON 030304 developmental biology 2. Zero hunger 0303 health sciences biology CATABOLITE REPRESSION 030306 microbiology SIGNAL-TRANSDUCTION NETWORK biology.organism_classification Culture Media Secretory protein Glucose extracellular proteome INDUCIBLE PROTEINS ARGININE TRANSLOCATION PATHWAY SECRETION |
| Zdroj: | Proteomics, 6(1), 268-281. Wiley |
| ISSN: | 1615-9853 |
| DOI: | 10.1002/pmic.200500091 |
| Popis: | The now finished genome sequence of Bacillus licheniformis DSM 13 allows the prediction of the genes involved in protein secretion into the extracellular environment as well as the prediction of the proteins which are translocated. From the sequence 296 proteins were predicted to contain an N-terminal signal peptide directing most of them to the Sec system, the main transport system in Gram-positive bacteria. Using 2-DE the extracellular proteome of B. licheniformis grown in different media was studied. From the approximately 200 spots visible on the gels, 89 were identified that either contain an N-terminal signal sequence or are known to be secreted by other mechanisms than the Sec pathway. The extracellular proteome of B. licheniformis includes proteins from different functional classes, like enzymes for the degradation of various macromolecules, proteins involved in cell wall turnover, flagellum- and phage-related proteins and some proteins of yet unknown function. Protein secretion is highest during stationary growth phase. Furthermore, cells grown in complex medium secrete considerably higher protein amounts than cells grown in minimal medium. Limitation of phosphate, carbon and nitrogen sources results in the secretion of specific proteins that may be involved in counteracting the starvation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text