The microRNA Signature in Response to Insulin Reveals Its Implication in the Transcriptional Action of Insulin in Human Skeletal Muscle and the Role of a Sterol Regulatory Element-Binding Protein-1c/Myocyte Enhancer Factor 2C Pathway
| Autor: | Etienne Lefai, Hubert Vidal, Sophie Rome, Rémi Rabasa-Lhoret, Jennifer Rieusset, Marie-Paule Gustin, Catherine Cerutti, Emmanuelle Meugnier, Emmanuel Disse, Aurélie Granjon, Christian Z. Paultre, Isabelle Güller, Martine Laville |
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| Přispěvatelé: | Cardiovasculaire, métabolisme, diabétologie et nutrition (CarMeN), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Hospices Civils de Lyon (HCL), Centre Hospitalier Lyon Sud [CHU - HCL] (CHLS), Hospices Civils de Lyon (HCL), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM), Vericel, Evelyne, Régulations métaboliques, nutrition et diabètes - UM55 (RMND UM55), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Agressions vasculaires et réponses tissulaires, Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM), Service de Diabétologie et Nutrition, Hospices Civils de Lyon (HCL)-Hôpital Edouard Herriot [CHU - HCL], Centre de Recherche en Nutrition Humaine Rhône-Alpes (CRNH-RA), Université de Lyon-Université de Lyon-CHU Grenoble-Hospices Civils de Lyon (HCL)-CHU Saint-Etienne-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Montreal Diabetes Research Center, Montreal University, This work was supported by INRA and INSERM and a grant from ALFEDIAM-Roche., Centre de Recherche en Nutrition Humaine Rhône-Alpes (CRNH-RH), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique (INRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Jean Monnet [Saint-Étienne] (UJM)-CHU Saint-Etienne-Hospices Civils de Lyon (HCL)-CHU Grenoble-Université Joseph Fourier - Grenoble 1 (UJF), Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Institut National de la Recherche Agronomique (INRA), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Hospices Civils de Lyon (HCL)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de la Recherche Agronomique (INRA), Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique (INRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Jean Monnet [Saint-Étienne] (UJM)-CHU Saint-Etienne-Hospices Civils de Lyon (HCL)-CHU Grenoble |
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Transcription
Genetic Biopsy Endocrinology Diabetes and Metabolism medicine.medical_treatment [SDV]Life Sciences [q-bio] Polymerase Chain Reaction MESH: Glucose Clamp Technique MESH: Down-Regulation MESH: Biopsy 0302 clinical medicine Reference Values Insulin Myocyte ComputingMilieux_MISCELLANEOUS Oligonucleotide Array Sequence Analysis 0303 health sciences MESH: Muscle Skeletal MEF2 Transcription Factors MESH: Reference Values MESH: Myogenic Regulatory Factors Glucose clamp technique MESH: Gene Expression Regulation Cell biology medicine.anatomical_structure Myogenic Regulatory Factors 030220 oncology & carcinogenesis MESH: Hyperinsulinism Original Article Sterol Regulatory Element Binding Protein 1 MESH: Diabetes Mellitus Type 2 medicine.medical_specialty DNA Complementary Down-Regulation MESH: Insulin Biology Cell Line 03 medical and health sciences MESH: Sterol Regulatory Element Binding Protein 1 Insulin resistance Downregulation and upregulation Hyperinsulinism Internal medicine Internal Medicine medicine [SDV.BBM] Life Sciences [q-bio]/Biochemistry Molecular Biology Humans [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Muscle Skeletal 030304 developmental biology MESH: Humans MESH: Transcription Genetic Skeletal muscle MESH: Polymerase Chain Reaction MESH: DNA Complementary medicine.disease MESH: Cell Line MicroRNAs Insulin receptor [SDV.AEN] Life Sciences [q-bio]/Food and Nutrition Metabolism Endocrinology Diabetes Mellitus Type 2 Gene Expression Regulation Hyperglycemia MESH: Oligonucleotide Array Sequence Analysis Glucose Clamp Technique biology.protein MESH: Hyperglycemia MESH: MicroRNAs [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition |
| Zdroj: | Diabetes Diabetes, American Diabetes Association, 2009, 58 (11), pp.2555-2564 Diabetes, 2009, 58 (11), pp.2555-64. ⟨10.2337/db09-0165⟩ Diabetes, American Diabetes Association, 2009, 58 (11), pp.2555-64. ⟨10.2337/db09-0165⟩ |
| ISSN: | 0012-1797 1939-327X |
| Popis: | OBJECTIVE Factors governing microRNA expressions in response to changes of cellular environment are still largely unknown. Our aim was to determine whether insulin, the major hormone controlling whole-body energy homeostasis, is involved in the regulation of microRNA expressions in human skeletal muscle. RESEARCH DESIGN AND METHODS We carried out comparative microRNA (miRNA) expression profiles in human skeletal muscle biopsies before and after a 3-h euglycemic-hyperinsulinemic clamp, with TaqMan low-density arrays. Then, using DNA microarrays, we determined the response to insulin of the miRNA putative target genes in order to determine their role in the transcriptional action of insulin. We further characterized the mechanism of action of insulin on two representative miRNAs, miR-1 and miR-133a, in human muscle cells. RESULTS Insulin downregulated the expressions of 39 distinct miRNAs in human skeletal muscle. Their potential target mRNAs coded for proteins that were mainly involved in insulin signaling and ubiquitination-mediated proteolysis. Bioinformatic analysis suggested that combinations of different downregulated miRNAs worked in concert to regulate gene expressions in response to insulin. We further demonstrated that sterol regulatory element–binding protein (SREBP)-1c and myocyte enhancer factor 2C were involved in the effect of insulin on miR-1 and miR-133a expression. Interestingly, we found an impaired regulation of miRNAs by insulin in the skeletal muscle of type 2 diabetic patients, likely as consequences of altered SREBP-1c activation. CONCLUSIONS This work demonstrates a new role of insulin in the regulation of miRNAs in human skeletal muscle and suggests a possible implication of these new modulators in insulin resistance. |
| Databáze: | OpenAIRE |
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