ClC-2 Activation Modulates Regulatory Volume Decrease
| Autor: | C. Li, E. Garami, M. Ramjeesingh, Y. Wang, Christine E. Bear, H. Xiong, K. Galley |
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| Rok vydání: | 1999 |
| Předmět: |
Cell type
Patch-Clamp Techniques Light Physiology Immunoblotting Biophysics Gene Expression Nerve Tissue Proteins Endogeny Sf9 4 4'-Diisothiocyanostilbene-2 2'-Disulfonic Acid In Vitro Techniques Spodoptera Transfection Chloride Antibodies Cell Line Meglumine Chloride Channels Osmotic Pressure medicine Animals Cell Size biology urogenital system Gramicidin Conductance Cell Biology Molecular biology Rats Cell biology CLC-2 Chloride Channels Perfusion Volume (thermodynamics) Nitrobenzoates Chloride channel biology.protein Antibody medicine.drug |
| Zdroj: | Journal of Membrane Biology. 167:215-221 |
| ISSN: | 1432-1424 0022-2631 |
| DOI: | 10.1007/s002329900485 |
| Popis: | ClC-2 belongs to a large family of chloride channels and its expression in certain cell types is associated with the appearance of swelling-activated chloride (Cl-) currents. In the present report, we examined the hypothesis that ClC-2 plays a role in regulatory volume decrease by expressing ClC-2 in Sf9 cells using the baculovirus system. First, we showed that ClC-2 protein expression is associated with appearance of a Cl- conductance which is activated by hypo-osmotic shock and can be distinguished from swelling-activated chloride currents endogenous to Sf9 cells on the basis of its pharmacology and specific inhibition by an anti-ClC-2 antibody. Second, we show that the rate of regulatory volume decrease is significantly enhanced in Sf9 cells expressing ClC-2 protein. Hence, our data support the hypothesis that ClC-2 is capable of mediating regulatory volume decrease. |
| Databáze: | OpenAIRE |
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