Two N-Terminal Domains of Kv4 K+ Channels Regulate Binding to and Modulation by KChIP1
| Autor: | Laura Lin, Mark Stahl, William S. Somers, Karen C. Carroll, Zhang-Bao Xu, Pranab K. Chanda, KeWei Wang, Karl Malakian, Robert Scannevin, Flora Jow, Meggin Taylor, Lydia Mosyak, Wade Edris, Alan H. Katz, Jennifer Megules, Stephane Olland, Kenneth J. Rhodes, Mark R. Bowlby, Weixin Xu, Qiang Lu, David C. Kopsco |
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| Rok vydání: | 2004 |
| Předmět: |
Models
Molecular Patch-Clamp Techniques Potassium Channels Neuroscience(all) Crystallography X-Ray Cell Line Membrane Potentials Calcium-binding protein Animals Humans Amino Acid Sequence K channels Binding Sites Chemistry General Neuroscience Calcium-Binding Proteins Cell Membrane Mutagenesis Kv Channel-Interacting Proteins Structural framework Protein Structure Tertiary Transport protein N-terminus Protein Subunits Shal Potassium Channels Terminal (electronics) Biochemistry Potassium Channels Voltage-Gated Mutagenesis Site-Directed Oocytes cardiovascular system Biophysics Protein Binding |
| Zdroj: | Neuron. 41:587-598 |
| ISSN: | 0896-6273 |
| Popis: | The family of calcium binding proteins called KChIPs associates with Kv4 family K + channels and modulates their biophysical properties. Here, using mutagenesis and X-ray crystallography, we explore the interaction between Kv4 subunits and KChIP1. Two regions in the Kv4.2 N terminus, residues 7–11 and 71–90, are necessary for KChIP1 modulation and interaction with Kv4.2. When inserted into the Kv1.2 N terminus, residues 71–90 of Kv4.2 are also sufficient to confer association with KChIP1. To provide a structural framework for these data, we solved the crystal structures of Kv4.3N and KChIP1 individually. Taken together with the mutagenesis data, the individual structures suggest that that the Kv4 N terminus is required for stable association with KChIP1, perhaps through a hydrophobic surface interaction, and that residues 71–90 in Kv4 subunits form a contact loop that mediates the specific association of KChIPs with Kv4 subunits. |
| Databáze: | OpenAIRE |
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