PKC-β activation inhibits IL-18-binding protein causing endothelial dysfunction and diabetic atherosclerosis
| Autor: | Pedro Geraldes, Marie-Odile Guimond, Martin Paré, Marie-Claude Durpès, Mark D. Rekhter, Raphaël Beland, Catherine Morin, Judith Paquin-Veillet, George L. King |
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| Rok vydání: | 2014 |
| Předmět: |
Male
medicine.medical_specialty Physiology medicine.medical_treatment Vascular Cell Adhesion Molecule-1 Biology Monocytes Ruboxistaurin Proinflammatory cytokine Diabetes Complications chemistry.chemical_compound Mice Apolipoproteins E Physiology (medical) Internal medicine Protein Kinase C beta medicine Diabetes Mellitus Animals Endothelial dysfunction Protein kinase C Cell adhesion molecule Monocyte Macrophages Interleukin-18 Endothelial Cells Original Articles medicine.disease Atherosclerosis Endocrinology medicine.anatomical_structure Cytokine chemistry Interleukin 18 Cardiology and Cardiovascular Medicine |
| Zdroj: | Cardiovascular research. 106(2) |
| ISSN: | 1755-3245 |
| Popis: | Aims Clinical observations showed a correlation between accelerated atherosclerosis in diabetes and high plasmatic level of IL-18, a pro-inflammatory cytokine. IL-18 enhances the production of inflammatory cytokines and cellular adhesion molecules contributing to atherosclerotic plaque formation and instability. Previous studies indicated that protein kinase C (PKC)-β inhibition prevented macrophage-induced cytokine expression involved in diabetic (DM) atherosclerotic plaque development. However, the role of PKC-β activation on IL-18/IL-18-binding protein (IL-18BP) pathway causing endothelial dysfunction and monocyte adhesion in diabetes has never been explored. Methods and results Apoe −/− mice were rendered DM and fed with western diet containing ruboxistaurin (RBX), a PKC-β inhibitor. After 20 weeks, atherosclerotic plaque composition was quantified. Compared with non-diabetic, DM mice exhibited elevated atherosclerotic plaque formation, cholestoryl ester content and macrophage infiltration, as well as reduced IL-18BP expression in the aorta which was prevented with RBX treatment. Endothelial cells (ECs) and macrophages were exposed to normal or high glucose (HG) levels with or without palmitate and recombinant IL-18 for 24 h. The combined HG and palmitate condition was required to increase IL-18 expression and secretion in macrophages, while it reduced IL-18BP expression in EC causing up-regulation of the vascular cell adhesion molecule (VCAM)-1 and monocyte adhesion. Elevated VCAM-1 expression and monocyte adherence were prevented by siRNA, RBX, and IL-18 neutralizing antibody. Conclusion Our study unrevealed a new mechanism by which PKC-β activation promotes EC dysfunction caused by the de-regulation of the IL-18/IL-18BP pathway, leading to increased VCAM-1 expression, monocyte/macrophage adhesion, and accelerated atherosclerotic plaque formation in diabetes. |
| Databáze: | OpenAIRE |
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