miR-26a and miR-214 down-regulate expression of the PTEN gene in chronic lymphocytic leukemia, but not PTEN mutation or promoter methylation
| Autor: | Run Zhang, Wei Xu, Li Wang, Jianyong Li, Zhi-Jian Zou, Ji Xu, Lei Fan, Tian Tian |
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| Rok vydání: | 2014 |
| Předmět: |
PTEN
Chronic lymphocytic leukemia Blotting Western Molecular Sequence Data Down-Regulation Polymorphism Single Nucleotide microRNA Tumor Cells Cultured medicine Humans Promoter Regions Genetic 3' Untranslated Regions Regulation of gene expression Base Sequence biology Gene Expression Regulation Leukemic Reverse Transcriptase Polymerase Chain Reaction PTEN Phosphohydrolase Sequence Analysis DNA Methylation DNA Methylation medicine.disease Leukemia Lymphocytic Chronic B-Cell Molecular biology Reverse transcription polymerase chain reaction MicroRNAs Leukemia Oncology Mutation DNA methylation Cancer research biology.protein chronic lymphocytic leukemia Tumor Suppressor Protein p53 Research Paper |
| Zdroj: | Oncotarget |
| ISSN: | 1949-2553 |
| DOI: | 10.18632/oncotarget.2626 |
| Popis: | We previous found the expression level of PTEN was low in the chronic lymphocytic leukemia (CLL) patients. To assess the pathogenic contribution of the low expression of PTEN, we determined PTEN-regulating miRNA interference, PTEN promoter methylation and PTEN gene mutation condition in CLL. One hundred and fifty-four previously untreated CLL patients and 200 cases of healthy controls were sequenced in exons 5−9 of PTEN. None of single nucleotide polymorphism site or mutation was detected in the coding sequences of those exons. Methylation of PTEN promoter was found in one (1.33%) of the 75 patients with CLL, but none of the 25 age-matched control subjects. We found that PTEN was a potential target of miR-26a and miR-214, which had been confirmed following dual-luciferase reporter assays, reverse transcription polymerase chain reaction and Western blotting. High expression of miR-26a was associated with advanced Binet stage (P=0.012), p53 aberrations (P=0.014) and inferior time to first treatment (P=0.038), and high expression of miR-214 was only associated with p53 aberrations (P=0.041). Inhibition of miR-26a or miR-214 could induce more apoptosis in primary cultured CLL cells. These findings support miR-26a and miR-214 down-regulate expression of PTEN in CLL, but not PTEN mutation or promoter methylation. |
| Databáze: | OpenAIRE |
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