The emergence of sequence-dependent structural motifs in stretched, torsionally constrained DNA
| Autor: | Agnes Noy, R. J. Greenall, Mark C. Leake, Jack W Shepherd, Matt Probert |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Base pair
Stacking Molecular Dynamics Simulation Biology 01 natural sciences Biophysical Phenomena DNA sequencing 03 medical and health sciences Molecular dynamics chemistry.chemical_compound 0103 physical sciences Genetics Structural motif Base Pairing 030304 developmental biology 0303 health sciences Base Sequence Molecular Structure 010304 chemical physics Chemistry Hydrogen bond Computational Biology Hydrogen Bonding Biomolecules (q-bio.BM) DNA GC Rich Sequence Quantitative Biology - Biomolecules FOS: Biological sciences Biophysics Nucleic Acid Conformation DNA supercoil |
| Zdroj: | Nucleic Acids Research |
| DOI: | 10.1101/599480 |
| Popis: | The double-helical structure of DNA results from canonical base pairing and stacking interactions. However, variations from steady-state conformations result from mechanical perturbations in cells. These different topologies have physiological relevance but their dependence on sequence remains unclear. Here, we use molecular dynamics simulations to show that sequence differences result in markedly different structural motifs upon physiological twisting and stretching. We simulated overextension on four different sequences of DNA ((AA)12, (AT)12, (GG)12and (GC)12) with supercoiling densities within the physiological range. We found that DNA denatures in the majority of stretching simulations, surprisingly including those with overtwisted DNA. GC-rich sequences were observed to be more stable than AT-rich, with the specific response dependent on base pair ordering. Furthermore, we found that (AT)12forms stable periodic structures with non-canonical hydrogen bonds in some regions and non-canonical stacking in others, whereas (GC)12forms a stacking motif of four base pairs independent of supercoiling density. Our results demonstrate that 20-30% DNA extension is sufficient for breaking B-DNA around and significantly above cellular supercoiling, and that the DNA sequence is crucial for understanding structural changes under mechanical stress. Our findings have important implications for the activities of protein machinery interacting with DNA in all cells. |
| Databáze: | OpenAIRE |
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