The contribution of cis- and trans-acting variants to gene regulation in wild and domesticated barley under cold stress and control conditions
| Autor: | Matthew Haas, Axel Himmelbach, Martin Mascher |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Crops
Agricultural 0106 biological sciences 0301 basic medicine Physiology Plant Science Biology 01 natural sciences Domestication Crop 03 medical and health sciences Allele-specific expression Gene Alleles Hordeum vulgare Hybrid Genetics Regulation of gene expression AcademicSubjects/SCI01210 Cold-Shock Response barley food and beverages RNA Hordeum Research Papers 030104 developmental biology Crop Molecular Genetics Regulatory sequence cold stress gene regulation 010606 plant biology & botany |
| Zdroj: | Journal of Experimental Botany |
| ISSN: | 1460-2431 0022-0957 |
| DOI: | 10.1093/jxb/eraa036 |
| Popis: | Barley, like other crops, has experienced a series of genetic changes that have impacted its architecture and growth habit to suit the needs of humans, termed the domestication syndrome. Domestication also resulted in a concomitant bottleneck that reduced sequence diversity in genes and regulatory regions. Little is known about regulatory changes resulting from domestication in barley. We used RNA sequencing to examine allele-specific expression in hybrids between wild and domesticated barley. Our results show that most genes have conserved regulation. In contrast to studies of allele-specific expression in interspecific hybrids, we find almost a complete absence of trans effects. We also find that cis regulation is largely stable in response to short-term cold stress. Our study has practical implications for crop improvement using wild relatives. Genes regulated in cis are more likely to be expressed in a new genetic background at the same level as in their native background. We present evidence for a near absence of trans effects controlling gene regulation in response to domestication and environmental stress |
| Databáze: | OpenAIRE |
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