SpL201: A conditionally immortalized Schwann cell precursor line that generates myelin

Autor: Beat Schweitzer, Verdon Taylor, Robin J.M. Franklin, Johanna Buchstaller, Peter Smith, Ueli Suter, Christian S. Lobsiger
Rok vydání: 2001
Předmět:
Cell Culture Techniques
Receptor
Nerve Growth Factor
Nestin
Rats
Sprague-Dawley
Myelin
Intermediate Filament Proteins
Peripheral myelin protein 22
Myelin Sheath
Cell Line
Transformed
Stem Cells
S100 Proteins
Cell Differentiation
Blood Proteins
Immunohistochemistry
Up-Regulation
Cell biology
medicine.anatomical_structure
Neurology
Neural Crest
Neuroglia
Myelin Proteins
Cell Survival
Schwann cell
Nerve Tissue Proteins
Receptors
Nerve Growth Factor
S100 Calcium Binding Protein beta Subunit
Biology
Cellular and Molecular Neuroscience
Precursor cell
Neurites
medicine
Animals
Cell Lineage
Nerve Growth Factors
Epidermal Growth Factor
Calcium-Binding Proteins
Colforsin
Fibroblasts
Antigens
Differentiation
Nerve Regeneration
Rats
Transplantation
Microscopy
Electron
nervous system
Cell culture
Culture Media
Conditioned
Octamer Transcription Factor-6
Schwann Cells
Mitogens
Neuroscience
Transcription Factors
Zdroj: Glia. 36:31-47
ISSN: 1098-1136
0894-1491
Popis: Dramatic progress has been made over recent years toward the elucidation of the mechanisms regulating lineage determination and cell survival in the developing peripheral nervous system. However, our understanding of Schwann cell development is limited. This is partly due to the difficulties in culturing primary Schwann cell precursor cells, the earliest developmental stage of the Schwann cell lineage defined to date. Both the inability to maintain cultured Schwann cell precursor cells in an undifferentiated state and the technical difficulties involved in their isolation have hampered progress. We have conditionally immortalized rat Schwann cell precursor cells using a retrovirally encoded EGFR/neu fusion protein to circumvent these problems and to generate a source of homogeneous cells. The resulting SpL201 cell line expresses p75 and nestin, two proteins expressed by neural crest-derived cells, as well as peripheral myelin protein 22, protein zero, and Oct-6 as markers of the Schwann cell lineage. When cultured in EGF-containing medium, the SpL201 cells proliferate and maintain an undifferentiated, Schwann cell precursor cell-like state. The cell line is dependent on EGF for survival but can differentiate into early Schwann cell-like cells in response to exogenous factors. Like primary rat Schwann cells, SpL201 cells upregulate Oct-6 and myelin gene expression in response to forskolin treatment. Furthermore, the SpL201 cell line can form myelin in the presence of axons in vitro and is capable of extensively remyelinating a CNS white matter lesion in vivo. Thus, this cell line provides a valuable and unique tool to study the Schwann cell lineage, including differentiation from the Schwann cell precursor cell stage through to myelination.
Databáze: OpenAIRE
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