Intercept-PCR, an Improvement for Elevating Performance to Find a New Member of a Certain Gene Family
| Autor: | Tatsuro Irimura, Michinari Hamaguchi, Satoru Matsuda, Yuji Nimura, Kou Miyazaki, Takahito Okuda, Tatsuyoshi Yamamoto, Yasukatu Ichigotani, Shigekazu Nakatsugawa |
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| Rok vydání: | 2000 |
| Předmět: |
DNA
Complementary Pcr cloning Bioengineering Computational biology Molecular cloning Biology Polymerase Chain Reaction Sensitivity and Specificity Applied Microbiology and Biotechnology Biochemistry Cell Line law.invention law Humans Gene family RNA Messenger Molecular Biology Gene Polymerase chain reaction DNA Primers Genetics Base Sequence Reverse Transcriptase Polymerase Chain Reaction Oligonucleotide Reproducibility of Results Reverse transcriptase Real-time polymerase chain reaction Multigene Family Biotechnology |
| Zdroj: | Molecular Biotechnology. 16:01-04 |
| ISSN: | 1073-6085 |
| Popis: | We have established a method by which the performance of reverse transcriptase coupled polymerase chain reaction (RT-PCR) for seeking a new gene is improved. The actual procedure is quite easy: it is only to add several specific oligonucleotides into the reaction mixture of the usual RT-PCR. To verify the effectiveness of this method is also easy: it is only to detect the PCR products in the preliminary experiment. The finding in the present study provides valuable information for gene cloning tactics. |
| Databáze: | OpenAIRE |
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