Multi-scale network imaging in a mouse model of amyloidosis
| Autor: | Nawal Zabouri, Samuel J. Barnes, Pavlina Pavlidi, Joseph Airey, Takaomi C. Saido, Leire Melgosa-Ecenarro, Carola I. Radulescu, Maksym V. Kopanitsa, Nazanin Doostdar, Takashi Saito |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male Dendritic spine Physiology Computer science Sensory system Mice Transgenic Neocortex Hippocampus 03 medical and health sciences Mice 0302 clinical medicine Calcium imaging Biological neural network medicine Animals Molecular Biology Cell Biology Amyloidosis Network dynamics Mice Inbred C57BL Disease Models Animal 030104 developmental biology medicine.anatomical_structure Microscopy Fluorescence Multiphoton Sensory maps Calcium Female Nerve Net Neuroscience 030217 neurology & neurosurgery Preclinical imaging |
| Zdroj: | Cell calcium. 95 |
| ISSN: | 1532-1991 |
| Popis: | The adult neocortex is not hard-wired but instead retains the capacity to reorganise across multiple spatial scales long into adulthood. Plastic reorganisation occurs at the level of mesoscopic sensory maps, functional neuronal assemblies and synaptic ensembles and is thought to be a critical feature of neuronal network function. Here, we describe a series of approaches that use calcium imaging to measure network reorganisation across multiple spatial scales in vivo. At the mesoscopic level, we demonstrate that sensory activity can be measured in animals undergoing longitudinal behavioural assessment involving automated touchscreen tasks. At the cellular level, we show that network dynamics can be longitudinally measured at both stable and transient functional assemblies. At the level of single synapses, we show that functional subcellular calcium imaging approaches can be used to measure synaptic ensembles of dendritic spines in vivo. Finally, we demonstrate that all three levels of imaging can be spatially related to local pathology in a preclinical rodent model of amyloidosis. We propose that multi-scale in vivo calcium imaging can be used to measure parallel plasticity processes operating across multiple spatial scales in both the healthy brain and preclinical models of disease. |
| Databáze: | OpenAIRE |
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