IMMUNOHISTOCHEMICAL STUDY OF THE CELLS INFILTRATING HUMAN RENAL ALLOGRAFTS BY THE ABC AND THE IGSS METHOD USING MONOCLONAL ANTIBODIES
| Autor: | Masashi Kodama, Kazuro Nishihara, Hirotaka Sako, K. Paku, H. Takayama, Kouji Okino, Tadao Tomoyoshi, Mitsuhiro Kawata, Hisao Yamada, Yoshihiro Nakane |
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| Rok vydání: | 1987 |
| Předmět: |
Adult
Graft Rejection Male Pathology medicine.medical_specialty Adolescent medicine.drug_class T cell H&E stain Inflammation Kidney Monoclonal antibody Monocytes Immunoenzyme Techniques medicine Humans Transplantation Homologous Lymphocytes Child Transplantation Staining and Labeling business.industry Macrophages Antibodies Monoclonal Middle Aged medicine.disease Kidney Transplantation medicine.anatomical_structure Antigens Surface Immunohistochemistry Female Gold medicine.symptom business Infiltration (medical) |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 0041-1337 |
| DOI: | 10.1097/00007890-198707000-00011 |
| Popis: | Sixty renal allograft tissues obtained from 29 patients were stained with hematoxylin and eosin. These tissues were histologically classified into 4 patterns according to the distribution pattern of the infiltrating cells: normal, focal, focal-diffuse, and diffuse types. Clinical signs of acute rejection were observed in 88% of the patients with the diffuse type infiltration, and 83% of those with the focal-diffuse type infiltration but in only 13% of those with the focal type infiltration. Twenty-four renal allografts were analyzed by the ABC and the IGSS methods using monoclonal antibodies. The number of T cells (Leu 1) accounted for about 80% of the total number of infiltrating cells; 2-8% of the cells were B cells (Leu 12); about 10% were NK/K cells (Leu 7); and 4-6% were monocytes/macrophages (Leu M3). As to helper/inducer T cell (Leu3a) and killer/suppressor T cell (Leu2a), which are T lymphocyte subsets, there were more Leu3a- than Leu2a-positive cells in focal type tissue, but there were more Leu2a- than Leu3a-positive cells in focal-diffuse and diffuse type tissue. In most cases that developed clinical signs of acute rejection, there were more Leu2a- than Leu3a-positive cells. The Leu3a/Leu2a ratio in most of the AZA-administered cases dropped immediately after the transplantation and maintained a low value, but in the CSA-administered cases it decreased gradually post-transplant. |
| Databáze: | OpenAIRE |
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