Prognostic Significance of Serum miR-22, miR-125b, and miR-15b in Non-Small Cell Lung Cancer Patients
| Autor: | Shang Ma, Yan-Jun Yin, Xin-Yong Zhang, Guang-Li Shi, Yan Chen, Wan-Qiu Bai |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Oncology
Male medicine.medical_specialty Lung Neoplasms medicine.medical_treatment Context (language use) General Biochemistry Genetics and Molecular Biology Biomarkers Pharmacological Internal medicine Carcinoma Non-Small-Cell Lung microRNA Antineoplastic Combined Chemotherapy Protocols Carcinoma Biomarkers Tumor Medicine Humans Lung cancer Survival analysis Neoplasm Staging Chemotherapy business.industry Gene Expression Profiling Middle Aged medicine.disease Prognosis Survival Analysis respiratory tract diseases MicroRNAs Real-time polymerase chain reaction Female Non small cell Drug Monitoring business |
| Zdroj: | Clinical laboratory. 66(6) |
| ISSN: | 1433-6510 |
| Popis: | Studies have shown that miRNA (miR) can be stably detected in serum, and aberrant expression of various miRNAs has shown diagnostic value in non-small cell lung cancer (NSCLC) patients. However, the role of miRNA in the context of prognosis has not been extensively investigated. Our previous study reported that miR-22, miR-125b, and miR-15b in serum had potential for use as tumor markers for auxiliary diagnosing of NSCLC. Therefore, the objective of this study was to detect the levels of miR-22, miR-125b, and miR-15b in serum from NSCLC patients and explore the potential prognostic significance of the three selected miRNAs.The relative expression of miR-22, miR-125b, and miR-15b in 74 patients with advanced NSCLC in pre- and post-chemotherapy were detected by real-time quantitative polymerase chain reaction.Serum level of miR-125b significantly decreased after chemotherapy (p0.05) and the levels of miR-15b significantly increased (p0.01), while there was no change in the level of serum miR-22 (Z = 0.716, p0.05). Compared with pre-chemotherapy, serum miR-125b expression in advanced NSCLC patients of responders (CR + PR) were significantly decreased post-chemotherapy (p0.05); serum miR-15b expression in advanced NSCLC patients of responders (CR + PR) were increased (p0.01). The chemotherapy sensitivity of advanced NSCLC patients with high expression of miR-125b was lower than that of NSCLC patients with low expression (p0.05). The chemotherapy sensitivity of advanced NSCLC patients with high expression of miR-15b was higher than that of NSCLC patients with low expression (p0.05). High levels of serum miR-125b and low levels of serum miR-15b were related to poor overall survival (p0.05).The serum levels of miR-125b and miR-15b in advanced NSCLC patients were changed pre- and post-chemotherapy and these changes were associated with chemotherapeutic response. Serum miR-125b and miR-15b have certain potential clinical value for chemotherapeutic response in advanced NSCLC. The serum levels of miR-125b and miR-15b in patients with advanced NSCLC before treatment may be used to estimate the overall survival. |
| Databáze: | OpenAIRE |
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