Genetic diversity ofBabesia bovisin virulent and attenuated strains
| Autor: | Monica L. Mazuz, B. Leibovitz, Varda Shkap, T. Molad, Ludmila Fleiderovitz, R. Wolkomirsky, L. Fish |
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| Rok vydání: | 2011 |
| Předmět: |
Protozoan Vaccines
Molecular Sequence Data Protozoan Proteins Virulence Vaccines Attenuated Microbiology Tandem repeat Babesiosis Escherichia coli Animals Parasite hosting Amino Acid Sequence Cloning Molecular Gene Genetics Cloning Genetic diversity Attenuated vaccine biology Reverse Transcriptase Polymerase Chain Reaction Genetic Variation Babesia bovis Sequence Analysis DNA biology.organism_classification Infectious Diseases Tandem Repeat Sequences Cattle Animal Science and Zoology Parasitology Sequence Alignment |
| Zdroj: | Parasitology. 139:317-323 |
| ISSN: | 1469-8161 0031-1820 |
| DOI: | 10.1017/s0031182011001995 |
| Popis: | SUMMARYThe aim of this study was to compare the genetic diversity of the single copyBv80gene sequences ofBabesia bovisin populations of attenuated and virulent parasites. PCR/ RT-PCR followed by cloning and sequence analyses of 4 attenuated and 4 virulent strains were performed. Multiple fragments in the range of 420 to 744 bp were amplified by PCR or RT-PCR. Cloning of the PCR fragments and sequence analyses revealed the presence of mixed subpopulations in either virulent or attenuated parasites with a total of 19 variants with 12 different sequences that differed in number and type of tandem repeats. High levels of intra- and inter-strain diversity of theBv80gene, with the presence of mixed populations of parasites were found in both the virulent field isolates and the attenuated vaccine strains. In addition, during the attenuation process, sequence analyses showed changes in the pattern of the parasite subpopulations. Despite high polymorphism found by sequence analyses, the patterns observed and the number of repeats, order, or motifs found could not discriminate between virulent field isolates and attenuated vaccine strains of the parasite. |
| Databáze: | OpenAIRE |
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