Identification of a Novel Prophage-Like Gene Cluster Actively Expressed in Both Virulent and Avirulent Strains of Leptospira interrogans Serovar Lai
| Autor: | Guoping Zhao, Bao-Yu Hu, Jinhong Qin, Qing Zhang, Yang Yang, Xiaokui Guo, Yi Zhong, Zhi-Ming Zhang |
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| Rok vydání: | 2008 |
| Předmět: |
Genomic Islands
Prophages Molecular Sequence Data Immunology Molecular Genomics Biology medicine.disease_cause Microbiology Bacterial Proteins Gene expression Gene cluster medicine Animals Humans Promoter Regions Genetic Gene Escherichia coli Prophage Oligonucleotide Array Sequence Analysis Genetics Regulation of gene expression Base Sequence Virulence Reverse Transcriptase Polymerase Chain Reaction Gene Expression Profiling Gene Expression Regulation Bacterial biology.organism_classification Molecular biology Gene expression profiling Infectious Diseases Multigene Family Parasitology Leptospira interrogans |
| Zdroj: | Infection and Immunity. 76:2411-2419 |
| ISSN: | 1098-5522 0019-9567 |
| DOI: | 10.1128/iai.01730-07 |
| Popis: | DNA microarray analysis was used to compare the differential gene expression profiles between Leptospira interrogans serovar Lai type strain 56601 and its corresponding attenuated strain IPAV. A 22-kb genomic island covering a cluster of 34 genes (i.e., genes LA0186 to LA0219) was actively expressed in both strains but concomitantly upregulated in strain 56601 in contrast to that of IPAV. Reverse transcription-PCR assays proved that the gene cluster comprised five transcripts. Gene annotation of this cluster revealed characteristics of a putative prophage-like remnant with at least 8 of 34 sequences encoding prophage-like proteins, of which the LA0195 protein is probably a putative prophage CI-like regulator. The transcription initiation activities of putative promoter-regulatory sequences of transcripts I, II, and III, all proximal to the LA0195 gene, were further analyzed in the Escherichia coli promoter probe vector pKK232-8 by assaying the reporter chloramphenicol acetyltransferase (CAT) activities. The strong promoter activities of both transcripts I and II indicated by the E. coli CAT assay were well correlated with the in vitro sequence-specific binding of the recombinant LA0195 protein to the corresponding promoter probes detected by the electrophoresis mobility shift assay. On the other hand, the promoter activity of transcript III was very low in E. coli and failed to show active binding to the LA0195 protein in vitro. These results suggested that the LA0195 protein is likely involved in the transcription of transcripts I and II. However, the identical complete DNA sequences of this prophage remnant from these two strains strongly suggests that possible regulatory factors or signal transduction systems residing outside of this region within the genome may be responsible for the differential expression profiling in these two strains. |
| Databáze: | OpenAIRE |
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