A 18F radiolabelled Zn(ii ) sensing fluorescent probe
| Autor: | George Firth, Justin Sturge, Michelle Kinnon, Nicholas J. Long, Thomas W. Price, Graeme J. Stasiuk, Charlotte J. Eling |
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| Rok vydání: | 2018 |
| Předmět: |
Fluorescence-lifetime imaging microscopy
010405 organic chemistry Chemistry Prostate cancer cell Metals and Alloys Quantum yield General Chemistry 010402 general chemistry Mass spectrometry 01 natural sciences Binding constant Fluorescence Catalysis 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials Yield (chemistry) Microscopy Materials Chemistry Ceramics and Composites Nuclear chemistry |
| Zdroj: | Price, T W, Firth, G, Eling, C J, Kinnon, M, Long, N J, Sturge, J & Stasiuk, G J 2018, ' A 18 F radiolabelled Zn(II) sensing fluorescent probe ', Chemical Communications, vol. 54, no. 26, pp. 3227-3230 . https://doi.org/10.1039/c8cc00687c |
| ISSN: | 1364-548X 1359-7345 |
| Popis: | A selective fluorescent probe for Zn(ii), AQA-F, has been synthesized. AQA-F exhibits a ratiometric shift in emission of up to 80 nm upon binding Zn(ii) ([AQA-F] = 0.1 mM, [Zn(ii)Cl2] = 0-300 μM). An enhancement of quantum yield from Φ = 4.2% to Φ = 35% is also observed. AQA-F has a binding constant, Kd = 15.2 μM with Zn(ii). This probe has been shown to respond to endogenous Zn(ii) levels in vitro in prostate and prostate cancer cell lines. [18F]AQA-F has been synthesized with a radiochemical yield of 8.6% and a radiochemical purity of 97% in 88 minutes. AQA-F shows the potential for a dual modal PET/fluorescence imaging probe for Zn(ii). |
| Databáze: | OpenAIRE |
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