Significantly improved oral uptake of amikacin in FVB mice in the presence of CRL-1605 copolymer
| Autor: | Martin R. Emanuele, Chinnaswamy Jagannath, Medea Mshvildadze, Robert L. Hunter, Alice Wells, Amitava Dasgupta, Eliud Sepulveda, Margaret Olsen |
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| Rok vydání: | 1999 |
| Předmět: |
Ethylene Oxide
Gram-negative bacteria Brush border Administration Oral Pharmacology General Biochemistry Genetics and Molecular Biology Microbiology Mice Surface-Active Agents otorhinolaryngologic diseases polycyclic compounds medicine Animals General Pharmacology Toxicology and Pharmaceutics Gram-Positive Cocci Amikacin biology medicine.diagnostic_test Chemistry Aminoglycoside General Medicine biochemical phenomena metabolism and nutrition bacterial infections and mycoses biology.organism_classification Anti-Bacterial Agents carbohydrates (lipids) Penicillin Intestinal Absorption Immunoassay Epoxy Compounds Female Efflux Pharmaceutical Vehicles medicine.drug |
| Zdroj: | Life Sciences. 64:1733-1738 |
| ISSN: | 0024-3205 |
| Popis: | Amikacin is an aminoglycoside which is used in the treatment of infection from gram negative bacteria. Amikacin is also used synergistically with penicillin against gram positive cocci. Amikacin cannot be delivered orally probably due to efflux of drug by P-glycoprotein pump in the brush border of intestine. We studied the possibility of delivering amikacin orally in mice using a copolymer (CRL-1605) as a vehicle. This copolymer inhibits P—glycoprotein pump. Two different doses of amikacin were used (500 mg/kg and 100 mg/kg). The concentration of polymer used was 132 mg/kg. The liquid formulation was fed to mice by gavage and serum amikacin concentrations were estimated after one hour and two hours using flourescence polarization immunoassay. We observed a two fold increase in serum amikacin concentration when amikacin was orally delivered in the presence of CRL-1605 compared to controls (amikacin alone). We conclude that gastrointestinal absorption of amikacin is significantly increased in the presence of CRL-1605 in mice. |
| Databáze: | OpenAIRE |
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