Short-term exercise training does not stimulate skeletal muscle ATP synthesis in relatives of humans with type 2 diabetes
| Autor: | Charlotte Ling, Marek Chmelik, Gertrud Kacerovsky-Bielesz, Michaela Kacerovsky, Michael Wolzt, Giovanni Pacini, Michael Roden, Gerhard Smekal, Leif Groop, Julia Szendroedi, Rochus Pokan, Michaela Farukuoye, Albrecht Ingo Schmid, Stephan Gruber, Ewald Moser |
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| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
medicine.medical_specialty
Endocrinology Diabetes and Metabolism 030209 endocrinology & metabolism Physical exercise Type 2 diabetes Biology Pathophysiology 03 medical and health sciences 0302 clinical medicine Insulin resistance Adenosine Triphosphate Internal medicine Surveys and Questionnaires Internal Medicine medicine Humans Family NADH NADPH Oxidoreductases Muscle Skeletal Exercise Heat-Shock Proteins 030304 developmental biology DNA Primers Adenosine Triphosphatases 0303 health sciences Electron Transport Complex I Polymorphism Genetic ATP synthase Skeletal muscle Feeding Behavior medicine.disease Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha medicine.anatomical_structure NDUFB6 Endocrinology Diabetes Mellitus Type 2 biology.protein Original Article PPARGC1A Gene polymorphism Transcription Factors |
| Zdroj: | Diabetes (N.Y.N.Y.) (2009). doi:10.2337/db08-1240 info:cnr-pdr/source/autori:Kacerovsky-Bielesz G.; Chmelik M.; Ling C.; Pokan R.; Szendroedi J.; Farukuoye M.; Kacerovsky M.; Schmid A. I.; Gruber S.; Wolzt M.; Moser E.; Pacini G.; Smekal G.; Groop L.; Roden, M./titolo:Short-term exercise training does not stimulate skeletal muscle ATP synthesis in relatives of humans with type 2 diabetes/doi:10.2337%2Fdb08-1240/rivista:Diabetes (N.Y.N.Y.)/anno:2009/pagina_da:/pagina_a:/intervallo_pagine:/volume Diabetes |
| DOI: | 10.2337/db08-1240 |
| Popis: | OBJECTIVE We tested the hypothesis that short-term exercise training improves hereditary insulin resistance by stimulating ATP synthesis and investigated associations with gene polymorphisms. RESEARCH DESIGN AND METHODS We studied 24 nonobese first-degree relatives of type 2 diabetic patients and 12 control subjects at rest and 48 h after three bouts of exercise. In addition to measurements of oxygen uptake and insulin sensitivity (oral glucose tolerance test), ectopic lipids and mitochondrial ATP synthesis were assessed using1H and31P magnetic resonance spectroscopy, respectively. They were genotyped for polymorphisms in genes regulating mitochondrial function, PPARGC1A (rs8192678) and NDUFB6 (rs540467). RESULTS Relatives had slightly lower (P = 0.012) insulin sensitivity than control subjects. In control subjects, ATP synthase flux rose by 18% (P = 0.0001), being 23% higher (P = 0.002) than that in relatives after exercise training. Relatives responding to exercise training with increased ATP synthesis (+19%, P = 0.009) showed improved insulin sensitivity (P = 0.009) compared with those whose insulin sensitivity did not improve. A polymorphism in the NDUFB6 gene from respiratory chain complex I related to ATP synthesis (P = 0.02) and insulin sensitivity response to exercise training (P = 0.05). ATP synthase flux correlated with O2uptake and insulin sensitivity. CONCLUSIONS The ability of short-term exercise to stimulate ATP production distinguished individuals with improved insulin sensitivity from those whose insulin sensitivity did not improve. In addition, the NDUFB6 gene polymorphism appeared to modulate this adaptation. This finding suggests that genes involved in mitochondrial function contribute to the response of ATP synthesis to exercise training. |
| Databáze: | OpenAIRE |
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