Functional ultrasound imaging of deep visual cortex in awake nonhuman primates
| Autor: | Pierre Pouget, José-Alain Sahel, Mathias Fink, Harry Ahnine, Frédéric Chavane, Fabrice Arcizet, Mickael Tanter, Kevin Blaize, Serge Picaud, Ulisse Ferrari, Marc Gesnik, Thomas Deffieux |
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| Přispěvatelé: | Institut de la Vision, Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Physique pour la médecine (PhysMed Paris), Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut du Cerveau = Paris Brain Institute (ICM), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Sorbonne Université (SU)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Institut de Neurosciences de la Timone (INT), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Institut Langevin - Ondes et Images (UMR7587) (IL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), ANR-10-LABX-0065,LIFESENSES,DES SENS POUR TOUTE LA VIE(2010), ANR-18-IAHU-0001,FOReSIGHT,Enabling Vision Restoration(2018), European Project: 610110,EC:FP7:ERC,ERC-2013-SyG,HELMHOLTZ(2014), Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre de recherche cerveau et cognition (CERCO), Institut des sciences du cerveau de Toulouse. (ISCT), Université Toulouse - Jean Jaurès (UT2J)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-CHU Toulouse [Toulouse]-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse - Jean Jaurès (UT2J)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-CHU Toulouse [Toulouse]-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Sorbonne Université (SU)-Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Paris (UP)-Centre National de la Recherche Scientifique (CNRS), Institut du Cerveau et de la Moëlle Epinière = Brain and Spine Institute (ICM), Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), University of Pittsburgh School of Medicine, Pennsylvania Commonwealth System of Higher Education (PCSHE), Fondation Ophtalmologique Adolphe de Rothschild [Paris], Université Paris sciences et lettres (PSL), Aix Marseille Université (AMU), Marazova, Katia, Pouget, Pierre |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Male
Brain activity and meditation Computer science [SDV]Life Sciences [q-bio] brain imaging nonhuman primate functional ultrasound imaging ocular dominance Ocular dominance 03 medical and health sciences [SPI]Engineering Sciences [physics] Spatio-Temporal Analysis Engineering 0302 clinical medicine Neuroimaging medicine Animals Wakefulness visual cortex Ultrasonography 030304 developmental biology Brain Mapping 0303 health sciences Multidisciplinary [SCCO.NEUR]Cognitive science/Neuroscience [SCCO.NEUR] Cognitive science/Neuroscience Reproducibility of Results Biological Sciences Macaca mulatta [SDV] Life Sciences [q-bio] Dominance Ocular Visual cortex medicine.anatomical_structure Physical Sciences Fixation (visual) Imaging technology Ultrasound imaging Female Spatiotemporal resolution Neuroscience Photic Stimulation 030217 neurology & neurosurgery |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America Proceedings of the National Academy of Sciences of the United States of America, 2020, 117, pp.14453-14463. ⟨10.1073/pnas.1916787117⟩ Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences, 2020, pp.201916787. ⟨10.1073/pnas.1916787117⟩ Proceedings of the National Academy of Sciences of the United States of America, 2020, 117 (25), pp.14453-14463. ⟨10.1073/pnas.1916787117⟩ Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences, 2020, 117 (25), pp.14453-14463. ⟨10.1073/pnas.1916787117⟩ |
| ISSN: | 0027-8424 1091-6490 |
| DOI: | 10.1073/pnas.1916787117⟩ |
| Popis: | Significance Nowadays, several techniques exist to study and better understand how the brain works (fMRI, EEG, electrophysiology, etc.). Each has its own advantages and disadvantages (spatiotemporal resolution, maximal recording depth, signal-to-noise ratio, etc.). In this article, we show that the new functional ultrasound (fUS) imaging technique is appropriate to record and map brain activity in awake primates on a scale previously unreachable. It allows distinguishing patterns similar to ocular dominance bands in the visual cortex through all layers of the cortex, which was impossible before with common techniques. This paper demonstrates the utility of fUS imaging for studying brain activity in awake primates and its interest to all neuroscientists. Deep regions of the brain are not easily accessible to investigation at the mesoscale level in awake animals or humans. We have recently developed a functional ultrasound (fUS) technique that enables imaging hemodynamic responses to visual tasks. Using fUS imaging on two awake nonhuman primates performing a passive fixation task, we constructed retinotopic maps at depth in the visual cortex (V1, V2, and V3) in the calcarine and lunate sulci. The maps could be acquired in a single-hour session with relatively few presentations of the stimuli. The spatial resolution of the technology is illustrated by mapping patterns similar to ocular dominance (OD) columns within superficial and deep layers of the primary visual cortex. These acquisitions using fUS suggested that OD selectivity is mostly present in layer IV but with extensions into layers II/III and V. This imaging technology provides a new mesoscale approach to the mapping of brain activity at high spatiotemporal resolution in awake subjects within the whole depth of the cortex. |
| Databáze: | OpenAIRE |
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