Microwave irradiation for rapid epidermis-dermis separation and improved epidermal cell immunodetection
| Autor: | Nilka L. Díaz, M. A. Sanchez, Félix J. Tapia |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Histology
integumentary system Immunoperoxidase Epidermis (botany) biology Analytical chemistry General Medicine Molecular biology Primary and secondary antibodies Medical Laboratory Technology medicine.anatomical_structure Antigen Dermis medicine biology.protein Immunohistochemistry Incubation Immunostaining |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1473-7760 1052-0295 |
| DOI: | 10.1080/bih.77.4.183.187 |
| Popis: | We explored the effects of microwave irradiation on epidermal-dermal separation and subsequent immunostaining of epidermal cells. Epidermal sheets were obtained after incubation in 0.02 M EDTA in PBS and microwave irradiation with 4 pulses of 420 watts for 5 sec, with a total incubation period of 4 min. The control epidermal sheets were immunostained for Langerhans cells and dendritic epidermal T cells using a conventional immunoperoxidase method. The experimental immunodetection of these cells was assisted by incubating the primary antibodies for 10 min at 70 watts. We showed a simple and rapid method for separation of the epidermal-dermal junction and immunostaining of epidermal cells with optimal morphological preservation. |
| Databáze: | OpenAIRE |
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