Rapid detection of Escherichia coli virulence factor genes using multiplex real-time TaqMan PCR assays
| Autor: | Donna West, K. A. Sprigings, Robert Davies, Claire Cassar, P. R. Wakeley, Jason Sawyer |
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| Rok vydání: | 2006 |
| Předmět: |
DNA
Bacterial Swine Virulence Factors Fimbria Virulence Cattle Diseases Biology medicine.disease_cause Microbiology Polymerase Chain Reaction Sensitivity and Specificity Virulence factor law.invention law Multiplex polymerase chain reaction TaqMan medicine Escherichia coli Animals Multiplex Polymerase chain reaction Escherichia coli Infections Swine Diseases General Veterinary Escherichia coli Proteins Gene Amplification General Medicine Molecular biology Fimbriae Bacterial Cattle Fimbriae Proteins |
| Zdroj: | Veterinary microbiology. 122(3-4) |
| ISSN: | 0378-1135 |
| Popis: | Three multiplex real-time TaqMan PCR assays were developed for the detection of Escherichia coli virulence factor genes in veterinary samples. Target virulence factors chosen were the fimbriae K88 (F4), K99 (F5), F41, F17, F18 and 987p (F6) and the toxins LT, STa and CDT IV. Detection of genes coding GAD were included in each assay as an internal control. These assays allow rapid identification of virulence factor genes using identical cycling conditions on an Mx3000Ptrade mark real-time PCR machine with the capacity to test up to 20 strains for 9 virulence genes in 1h. |
| Databáze: | OpenAIRE |
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