Fas-induced programmed cell death is mediated by a Ras-regulated O2- synthesis
| Autor: | H. Heinle, Erich Gulbins, K. Schlottmann, Otwin Linderkamp, U. Koppenhoefer, K. M. Coggeshall, Birgit Brenner, F. Lang, J. Welsch |
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| Rok vydání: | 1996 |
| Předmět: |
Programmed cell death
Ceramide Immunology Apoptosis Oncogene Protein p21(ras) Biology Transfection Inhibitory postsynaptic potential Antioxidants Cyclic N-Oxides Jurkat Cells chemistry.chemical_compound Anti-apoptotic Ras signalling cascade Humans Immunology and Allergy fas Receptor Intracellular signalling Fas receptor Acetylcysteine Cell biology Gene Expression Regulation chemistry Nitrogen Oxides Reactive Oxygen Species Research Article |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1365-2567 0019-2805 |
| Popis: | Fas induces apoptosis in lymphocytes via a poorly defined intracellular signalling cascade. Previously, we have demonstrated the involvement and significance of a signalling cascade from the Fas receptor via sphingomyelinases and ceramide to Ras in Fas-induced apoptosis. Here we demonstrate rapid and transient synthesis of reactive oxygen intermediates (ROI) via activation of Ras after Fas. Genetic inhibition of Ras by transfection of transdominant inhibitory N17Ras blocked Fas-mediated ROI synthesis and programmed cell death. Likewise, the antioxidants N-acetyl-cysteine and N-t-butyl-phenylnitrone abolished Fas-induced cell death, pointing to an important role for Ras-triggered ROI synthesis in Fas-mediated programmed cell death. |
| Databáze: | OpenAIRE |
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