Follicular fluid contents as predictors of success of in-vitro fertilization-embryo transfer
| Autor: | Ron Rabinowitz, M. Lancet, B.C. Adelmann-Grill, Neri Laufer, Aby Lewin, J.G. Shenker, N. Reich, E. Rom, Alex Tsafriri, B. Pevsner, R. Miskin |
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| Rok vydání: | 1987 |
| Předmět: |
medicine.medical_specialty
Cleavage Stage Ovum medicine.medical_treatment Alpha (ethology) Fertilization in Vitro Biology Plasminogen Activators Human fertilization Ovarian Follicle Internal medicine Follicular phase medicine Humans alpha-Macroglobulins Progesterone Pregnancy In vitro fertilisation Rehabilitation Obstetrics and Gynecology Embryo Transfer medicine.disease Follicular fluid Embryo transfer Microbial Collagenase Endocrinology Reproductive Medicine Female Plasminogen activator |
| Zdroj: | Human Reproduction. 2:505-510 |
| ISSN: | 1460-2350 0268-1161 |
| DOI: | 10.1093/oxfordjournals.humrep.a136579 |
| Popis: | In order to establish criteria for selection of the best ova in in-vitro fertilization-embryo transfer (IVF-ET) programes we have examined the follicular fluid (FF) levels of plasminogen activator (PA), collagenolytic activity, progesterone (P) and alpha 2 macroglobulin (alpha 2M) and related them to the success of pregnancy. PA activity was similar in FF of pregnant and nonpregnant cycles, 13.8 +/- 3.9 mU/ml versus 14.6 +/- 2.9 (mean +/- SEM) respectively. By contrast, FF from pregnant cycles exhibited lower collagenolytic activity (49.6 +/- 3.9% versus 67.9 +/- 3.0; P less than 0.001). Likewise, in a semi-quantitative assay of alpha 2M, only 18.4% of the aspirates from pregnant cycles showed a precipitation line, whereas 76.8% of those from non-pregnant cycles were positive. Levels of P in aspirates from pregnant cycles were in the intermediate range, as compared with those from non-pregnant cycles (0.06-5.5 micrograms/ml versus 0.02-12.0 micrograms/ml). All these assays can be completed before ET and performed in IVF-ET programmes. In conclusion, it seems that a combination of follicular alpha 2M levels and collagenolytic activity, and to a lesser extent addition of P assay, may serve as good criteria for selecting the best embryos for establishment of pregnancy. |
| Databáze: | OpenAIRE |
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