Opuntia humifusa Ameliorated Cerulein-Induced Acute Pancreatitis
| Autor: | Kyoung-Chel Park, Min-Jun Seo, Yong Kook Shin, Il-Joo Jo, Seung-Hee Seo, Ho-Joon Song, Jong Jin Kim, Hyuncheol Oh, Gi-Sang Bae, Sung-Joo Park, Jin-Han Park, Kyung Seuk Song, Youn-Chul Kim, Sun Bok Choi, Dong-Sung Lee |
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| Rok vydání: | 2014 |
| Předmět: |
medicine.medical_specialty
Time Factors Cell Survival Endocrinology Diabetes and Metabolism medicine.medical_treatment Blotting Western Intraperitoneal injection Gene Expression Apoptosis Acinar Cells Gastroenterology Mice Endocrinology Internal medicine Internal Medicine medicine Animals HMGB1 Protein Pancreas Cells Cultured Dose-Response Relationship Drug Hepatology Plant Extracts Reverse Transcriptase Polymerase Chain Reaction business.industry Opuntia Lipase medicine.disease Cholecystokinin Analog Mice Inbred C57BL Pancreatitis Acute Disease Amylases Cytokines Acute pancreatitis Female Mitogen-Activated Protein Kinases business Ceruletide Injections Intraperitoneal |
| Zdroj: | Pancreas. 43:118-127 |
| ISSN: | 0885-3177 |
| DOI: | 10.1097/mpa.0b013e318296f903 |
| Popis: | The aim of this study was to evaluate the effects of Opuntia humifusa (OH) on cerulein-induced acute pancreatitis (AP).Acute pancreatitis was induced via intraperitoneal injection of cholecystokinin analog cerulein (50 μg/kg). In the OH pretreatment group, OH was administered intraperitoneally (100, 250, or 500 mg/kg) 1 hour before first cerulein injection. In the posttreatment group, OH was administered intraperitoneally (500 mg/kg) 1 hour after the first cerulein injection. Furthermore, we isolated the pancreatic acinar cells using collagenase method, then investigated the acinar cell viability, cytokine productions, and the regulating mechanisms.The both pretreatment and posttreatment of OH treatment attenuated the severity of AP, as shown by the histology of the pancreas and lung, and inhibited neutrophil infiltration; serum amylase and lipase activities; proinflammatory cytokine expression such as interleukin 1, interleukin 6, and tumor necrosis factor α; and cell death including apoptosis and necrosis. Furthermore, OH inhibited the activation of c-Jun N-terminal kinases.These results suggest that OH reduces the severity of AP by inhibiting acinar cell death through c-Jun N-terminal kinases. |
| Databáze: | OpenAIRE |
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