Mutation Analysis in Cultured Cells of Transgenic Rodents
| Autor: | Stella Tommasi, Albert Zheng, Steven E. Bates, Ahmad Besaratinia |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
cII transgene mouse embryonic fibroblasts DNA Mutational Analysis Statistics as Topic Mutant Review Inbred C57BL Transgenic lcsh:Chemistry Mice Mutation Rate lcsh:QH301-705.5 Cells Cultured Spectroscopy Cultured General Medicine Computer Science Applications Physical Carcinogens carcinogen Embryo Mutation (genetic algorithm) Cells Transgene Mice Transgenic Computational biology Biology Catalysis Inorganic Chemistry 03 medical and health sciences In vivo Genetics Animals Physical and Theoretical Chemistry Molecular Biology Chemical Physics Mammalian Organic Chemistry Fibroblasts assay Embryo Mammalian In vitro Mice Inbred C57BL 030104 developmental biology lcsh:Biology (General) lcsh:QD1-999 Cell culture Mutation Mutation testing Other Biological Sciences mutation Other Chemical Sciences |
| Zdroj: | International Journal of Molecular Sciences, Vol 19, Iss 1, p 262 (2018) International journal of molecular sciences, vol 19, iss 1 Besaratinia, A; Zheng, A; Bates, SE; & Tommasi, S. (2018). Mutation Analysis in Cultured Cells of Transgenic Rodents.. International journal of molecular sciences, 19(1). doi: 10.3390/ijms19010262. UC Office of the President: Research Grants Program Office (RGPO). Retrieved from: http://www.escholarship.org/uc/item/5869t6tg International Journal of Molecular Sciences |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms19010262. |
| Popis: | To comply with guiding principles for the ethical use of animals for experimental research, the field of mutation research has witnessed a shift of interest from large-scale in vivo animal experiments to small-sized in vitro studies. Mutation assays in cultured cells of transgenic rodents constitute, in many ways, viable alternatives to in vivo mutagenicity experiments in the corresponding animals. A variety of transgenic rodent cell culture models and mutation detection systems have been developed for mutagenicity testing of carcinogens. Of these, transgenic Big Blue® (Stratagene Corp., La Jolla, CA, USA, acquired by Agilent Technologies Inc., Santa Clara, CA, USA, BioReliance/Sigma-Aldrich Corp., Darmstadt, Germany) mouse embryonic fibroblasts and the λ Select cII Mutation Detection System have been used by many research groups to investigate the mutagenic effects of a wide range of chemical and/or physical carcinogens. Here, we review techniques and principles involved in preparation and culturing of Big Blue® mouse embryonic fibroblasts, treatment in vitro with chemical/physical agent(s) of interest, determination of the cII mutant frequency by the λ Select cII assay and establishment of the mutation spectrum by DNA sequencing. We describe various approaches for data analysis and interpretation of the results. Furthermore, we highlight representative studies in which the Big Blue® mouse cell culture model and the λ Select cII assay have been used for mutagenicity testing of diverse carcinogens. We delineate the advantages of this approach and discuss its limitations, while underscoring auxiliary methods, where applicable. |
| Databáze: | OpenAIRE |
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