Genome-wide transcriptome analysis of human epidermal melanocytes
| Autor: | Elena Oancea, Kirk D. Haltaufderhyde |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
SLC45A2
RNA-Seq Skin Pigmentation Melanocyte Article Ion Channels Cell Line Transcriptome 03 medical and health sciences 0302 clinical medicine Genetics medicine Humans Gene 030304 developmental biology 0303 health sciences biology Genome Human Gene Expression Profiling Phenotype Gene expression profiling medicine.anatomical_structure Epidermal Cells 030220 oncology & carcinogenesis biology.protein Melanocytes Epidermis Transcription Factor Gene Signal Transduction Transcription Factors |
| Popis: | Because human epidermal melanocytes (HEMs) provide critical protection against skin cancer, sunburn, and photoaging, a genome-wide perspective of gene expression in these cells is vital to understanding human skin physiology. In this study we performed high throughput sequencing of HEMs to obtain a complete data set of transcript sizes, abundances, and splicing. As expected, we found that melanocyte specific genes that function in pigmentation were among the highest expressed genes. We analyzed receptor, ion channel and transcription factor gene families to get a better understanding of the cell signalling pathways used by melanocytes. We also performed a comparative transcriptomic analysis of lightly versus darkly pigmented HEMs and found 16 genes differentially expressed in the two pigmentation phenotypes; of those, only one putative melanosomal transporter (SLC45A2) has known function in pigmentation. In addition, we found 166 genes with splice isoforms expressed exclusively in one pigmentation phenotype, 17 of which are genes involved in signal transduction. Our melanocyte transcriptome study provides a comprehensive view and may help identify novel pigmentation genes and potential pharmacological targets. |
| Databáze: | OpenAIRE |
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