Molecular characterization of human 4Ig-B7-H3, a member of the B7 family with four Ig-like domains
| Autor: | Gerhard J. Zlabinger, Sophia Derdak, Peter Steinberger, Johannes Stöckl, Walter Knapp, Winfried F. Pickl, Christoph Klauser, Katharina Pfistershammer, Stefanie Kirchberger, Otto Majdic |
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| Rok vydání: | 2004 |
| Předmět: |
B7 Antigens
CD3 Complex T cell T-Lymphocytes Immunology Molecular Sequence Data Immunoglobulins Biology Ligands Lymphocyte Activation Monocytes Cell Line Antigen-Antibody Reactions Membrane Microdomains Antigens CD Complementary DNA medicine Immunology and Allergy Humans Amino Acid Sequence RNA Messenger Cloning Molecular Receptors Immunologic Messenger RNA Base Sequence Reverse Transcriptase Polymerase Chain Reaction Antibodies Monoclonal Dendritic Cells Sequence Analysis DNA Molecular biology In vitro Cell biology Protein Structure Tertiary Blot Molecular Weight medicine.anatomical_structure Membrane protein Organ Specificity Receptor-CD3 Complex Antigen T-Cell Multigene Family Expression cloning B7-1 Antigen Function (biology) Signal Transduction |
| Zdroj: | Journal of immunology (Baltimore, Md. : 1950). 172(4) |
| ISSN: | 0022-1767 |
| Popis: | In an effort to characterize molecules with immunoregulatory potential, we raised mAbs to human dendritic cells. We selected an Ab that recognizes a molecule that is induced on monocytes differentiated in vitro toward dendritic cells. Retroviral expression cloning identified this molecule as B7-H3, a member of the B7 family described recently. In contrast to an earlier report, in which B7-H3 was described as a molecule consisting of two Ig-like domains, our cDNA encoded a type I membrane protein with four Ig-like domains, and the molecule identified by us was therefore named 4Ig-B7-H3. mRNA analysis as well as Western blotting experiments performed by us did not reveal evidence for a small B7-H3. B7-H3 is not expressed on peripheral blood lymphocytes, monocytes, or granulocytes. Upon in vitro stimulation, the expression of B7-H3 is induced on T cells, B cells, and NK cells. A number of different approaches were used to investigate the function of human B7-H3. In contrast to an earlier report, our data do not support a costimulatory role of B7-H3 in anti-CD3-mediated activation of the TCR-complex resulting in T cell proliferation and IFN-γ production. |
| Databáze: | OpenAIRE |
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