Type I interferon-mediated immune response against influenza A virus is attenuated in the absence of p53
| Autor: | Zixue Shi, Beibei Li, Ke Liu, Yafeng Qiu, Jianchao Wei, Donghua Shao, Haixue Zheng, Yifan Yang, Zixiang Zhu, Zhiyong Ma |
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| Rok vydání: | 2014 |
| Předmět: |
Biophysics
Gene Expression Stimulation Chick Embryo Interferon alpha-2 Biology Virus Replication medicine.disease_cause Antiviral Agents Biochemistry Cell Line Dogs Immune system RNA interference Interferon Influenza A virus medicine Animals Humans Secretion RNA Small Interfering Molecular Biology Gene Interferon-alpha Cell Biology Genes p53 ISG15 Virology Recombinant Proteins Gene Knockdown Techniques Host-Pathogen Interactions Interferon Type I Tumor Suppressor Protein p53 medicine.drug |
| Zdroj: | Biochemical and Biophysical Research Communications. 454:189-195 |
| ISSN: | 0006-291X |
| Popis: | Influenza A virus (IAV) infection induces secretion of type I interferon (IFN) and activation of p53, which play essential roles in the host defense against tumor development and viral infection. In this study, we knocked down p53 expression by RNA interference. The expression levels of IFN-stimulated genes (ISGs) including IFN regulatory factor (IRF) 5, IRF9, ISG15, ISG20, guanylate-binding protein 1, retinoic acid-inducible gene-I and 2'-5'-oligoadenylate synthetase 1 were significantly attenuated in response to IAV infection and IFN-α stimulation in p53-knockdown cells. This attenuated expression of ISGs was associated with enhanced replication of IAV. Pretreatment of p53-knockdown cells with IFN-α failed to inhibit IAV replication, indicating impaired antiviral activity. These findings indicate that p53 plays an essential role in the enhancement of the type I IFN-mediated immune response against IAV infection. |
| Databáze: | OpenAIRE |
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