An antitumorigenic role for the IL-33 receptor, ST2L, in colon cancer
| Autor: | Charlotte O'Donnell, Aileen Houston, Sinead Carey, Jonathan Keane, Michael W. Bennett, Carola T. Murphy, Elizabeth Brint, Declan White, Micheal O'Riordain, Amr Mahmoud |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Serum Cancer Research Chemokine Colorectal cancer medicine.medical_treatment Expression Metastasis Mice 0302 clinical medicine Cell Movement Cytotoxic T cell Mice Inbred BALB C Gene knockdown Prognostic factor biology Colitis Cell biology Colon cancer Interleukin 10 Cytokine colon cancer Oncology 030220 oncology & carcinogenesis Colonic Neoplasms Effector Female CD8(+) T-Cells Receptors Cell Surface 03 medical and health sciences Interleukin-4 receptor medicine Hepatocellular-carcinoma Animals Humans Neoplasm Invasiveness IL-33 ST2 Cell Proliferation Inflammation Macrophages ST2 medicine.disease Interleukin-33 Interleukin-1 Receptor-Like 1 Protein 030104 developmental biology inflammation IL-33 biology.protein Cancer research Neoplasm Grading Translational Therapeutics |
| Zdroj: | British Journal of Cancer |
| Popis: | Background: Despite the importance of inflammation in cancer, the role of the cytokine IL-33, and its receptor ST2, in colon cancer is unclear. The aim of this study was to investigate the role of IL-33, and its receptor isoforms (ST2 and ST2L), in colon cancer. Methods: Serum levels of IL-33 and sST2 were determined with ELISA. ST2 and IL-33 expression was detected with quantitative real-time PCR (qRT–PCR), western blotting and immunohistochemistry. ST2 expression in CT26 cells was stably suppressed using ST2-specific shRNA. Cytokine and chemokine gene expression was detected with qRT–PCR. Results: Human colon tumours showed lower expression of ST2L as compared with adjacent non-tumour tissue (P |
| Databáze: | OpenAIRE |
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