Unique RNA 2 sequences of two Brazilian isolates of Pepper ringspot virus, a tobravirus
| Autor: | Tatsuya Nagata, Raquel Medeiros Vasques, Alice K. Inoue-Nagata, Adriana Ribeiro Silva Batista, Cícero Nicolini, Kelly Barreto Rodrigues, Mônica Alves de Macêdo, Fernando L. Melo |
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| Rok vydání: | 2014 |
| Předmět: |
Sequence analysis
Molecular Sequence Data Sequence Homology Plant Viruses Open Reading Frames Viral Proteins Solanum lycopersicum Virology Genetics Direct repeat RNA Viruses Molecular Biology Gene Plant Diseases biology RNA General Medicine Sequence Analysis DNA Virgaviridae biology.organism_classification Open reading frame Tobacco rattle virus RNA Viral Tobravirus Brazil |
| Zdroj: | Virus genes. 49(1) |
| ISSN: | 1572-994X |
| Popis: | Pepper ringspot virus (PepRSV) is a tobravirus reported only in Brazil. Here, the sequences of the complete RNA 2 segments and the 3′ end of the RNA 1 genomic regions of two new isolates from tomato plants were analyzed. The main ORF encodes the CP gene as other tobraviruses and termed ORF 1 of RNA 2. The second ORF was found only in one of the new isolates, although this gene was absent in the type isolate, CAM (collected in the 1960’s). Interestingly, this ORF 2 gene did not show any nucleotide and amino acid sequence similarities with known 2b genes of tobraviruses, an essential gene of tobraviruses for nematodes-transmission. The 5′UTR sequence of RNA 2 segment of CAM isolate was previously reported showing two impaired direct repeats; however, the direct-repeats were absent in these new isolates. An additional ORF was predicted upstream of the CP gene. This putative protein possessed a transmembrane domain similar to the ORFN1 of RNA 2 of Tobacco rattle virus SYM isolate, although there was no sequence similarity. This is the first report on the diversity of the RNA 2 sequences of PepRSV. |
| Databáze: | OpenAIRE |
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