SIRT1 inhibits monocyte adhesion to the vascular endothelium by suppressing Mac-1 expression on monocytes
Autor: | Chi Dae Kim, Seung Jin Lee, Min A. Jang, Seung Eun Baek |
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Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
0301 basic medicine
endocrine system diseases THP-1 Cells Transcriptional regulatory elements Clinical Biochemistry lcsh:Medicine Biochemistry Monocytes 0302 clinical medicine Sirtuin 1 THP1 cell line lcsh:QD415-436 Lipid signalling biology Chemistry NF-kappa B Chronic inflammation Adhesion Flow Cytometry CREB-Binding Protein Immunohistochemistry Cell biology Mechanisms of disease medicine.anatomical_structure 030220 oncology & carcinogenesis Molecular Medicine lipids (amino acids peptides and proteins) biological phenomena cell phenomena and immunity Signal transduction hormones hormone substitutes and hormone antagonists Chromatin Immunoprecipitation Endothelium Transgene Blotting Western CREB Peripheral blood mononuclear cell Article lcsh:Biochemistry 03 medical and health sciences Human Umbilical Vein Endothelial Cells medicine Humans Molecular Biology lcsh:R Atherosclerosis Toll-Like Receptor 2 enzymes and coenzymes (carbohydrates) TLR2 030104 developmental biology Mutagenesis Site-Directed biology.protein Endothelium Vascular |
Zdroj: | Experimental and Molecular Medicine, Vol 51, Iss 4, Pp 1-12 (2019) Experimental & Molecular Medicine |
ISSN: | 2092-6413 1226-3613 |
Popis: | SIRT1 signaling pathways modulate vascular inflammation; however, the precise role of SIRT1 in monocyte adhesion to the vascular endothelium, a key event initiating vascular inflammation, is unclear. Thus, this study investigated the roles and molecular interaction of SIRT1 and TLR2 in regulating monocyte adhesion to the vascular endothelium. In vitro, both Mac-1 expression and the endothelial adhesion of THP-1 cells stimulated with Pam3CSK4, a TLR2 ligand, were markedly increased in association with a decreased expression of SIRT1. In THP-1 cells stimulated with Pam3CSK4, the promoter activity and expression of SIRT1 were decreased. The TLR2-dependent suppression of SIRT1 expression in THP-1 cells was mediated by the transcription factors NF-κB and CREB, suggesting that the TLR2-mediated NF-κB and CREB signaling downregulated SIRT1 expression in monocytes. In peripheral blood monocytes (PBMCs) isolated from SIRT1 transgenic (TG) mice and THP-1 cells treated with recombinant SIRT1, both the increased Mac-1 expression and endothelial adhesion induced by Pam3CSK4 were significantly attenuated. In addition, the en face immunohistochemical study showed a marked increase in monocyte adhesion to the aortic endothelium of WT mice treated with Pam3CSK4, which was significantly attenuated in Pam3CSK4-treated SIRT1 TG mice. Moreover, a greater number of atherosclerotic plaques formed in WT mice fed a high-fat diet than in SIRT1 TG mice, indicating a pivotal role for SIRT1 in preventing vascular inflammation. Based on these results, SIRT1 might be a potential target for researchers aiming to develop therapeutic interventions for vascular inflammation, including atherosclerosis. Atherosclerosis: Intervening in the early stages of plaque formation Researchers have identified a possible new tool, the signaling molecule SIRT1, to fight vascular diseases such as atherosclerosis, hardening of the arteries. In atherosclerosis, plaque builds up in arteries, restricting blood flow and potentially causing heart attack or stroke. Early in the disease, white blood cells called monocytes stick to artery walls, triggering inflammation. Administering SIRT1 was known to decrease inflammation, but how it did so was unclear. Chi Dae Kim at Pusan National University in South Korea and co-workers investigated the signals that trigger monocytes to attach to artery walls, and how SIRT1 might prevent attachment. After identifying the signals, further testing in mice fed a high-fat diet showed that SIRT1 strongly decreased plaque formation. These findings illuminate the causes and progression of atherosclerosis and may help identify better treatments. |
Databáze: | OpenAIRE |
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