Synergic in vitro combinations of artemisinin, pyrimethamine and methylene blue against Neospora caninum
| Autor: | Gabriela de Luca, Cássia Mariana Bronzon da Costa, Ana Patrícia Yatsuda, Luiz Miguel Pereira, Nathália de Lima Martins Abichabki |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
In Vitro Techniques Pharmacology Apicomplexa Antimalarials 03 medical and health sciences chemistry.chemical_compound Chlorocebus aethiops parasitic diseases medicine Animals Artemisinin Vero Cells chemistry.chemical_classification Reactive oxygen species General Veterinary biology Coccidiosis Neospora Drug Synergism General Medicine biology.organism_classification Artemisinins Neospora caninum In vitro Methylene Blue Drug repositioning Pyrimethamine 030104 developmental biology chemistry Drug Therapy Combination Parasitology Methylene blue medicine.drug |
| Zdroj: | Veterinary Parasitology. 249:92-97 |
| ISSN: | 0304-4017 |
| DOI: | 10.1016/j.vetpar.2017.11.014 |
| Popis: | Neospora caninum is a member of Apicomplexa phylum, the causative agent of neosporosis. The neosporosis combat is not well established and several strategies related to vaccine, chemotherapy and immune modulation are under development. In this work, we evaluated the effects of artemisinin (Art), methylene blue (MB) and pyrimethamine (Pyr) alone or associated, on N. caninum proliferation and elimination using LacZ tagged tachyzoites. The reactive oxygen species (ROS) production after incubation with Art were also performed. Our results indicate that combinations of classical antimalarial drugs improve the parasite control, allowing the use of three drugs in a single dose. Additionally, artemisinin demonstrated distinct ROS production patterns in intra and extracellular N. caninum forms. The drug repurposing appears as a suitable approach, allowing a fast and safe method to evaluate old drugs but novel candidates against neosporosis. |
| Databáze: | OpenAIRE |
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