Localization of phosphotyrosine adaptor protein ShcD/SHC4 in the adult rat central nervous system

Autor: Hayley R. Lau, Hannah N. Robeson, Jasmin Lalonde, Laura A. New, Nina Jones, John N. Armstrong
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Olfactory system
Central Nervous System
Male
Cerebellum
Src Homology 2 Domain-Containing
Transforming Protein 2
ShcB
Src Homology 2 Domain-Containing
Transforming Protein 3
Central nervous system
ShcD
ShcC
Biology
lcsh:RC321-571
Subgranular zone
Rats
Sprague-Dawley
03 medical and health sciences
Cellular and Molecular Neuroscience
0302 clinical medicine
Olfactory bulb
Olfactory nerve
Neural Stem Cells
Shc4
medicine
Animals
Neural progenitor cells
lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry
030304 developmental biology
Neurons
0303 health sciences
General Neuroscience
lcsh:QP351-495
Signal transducing adaptor protein
Immunohistochemistry
Neural stem cell
Cell biology
lcsh:Neurophysiology and neuropsychology
medicine.anatomical_structure
nervous system
Shc Signaling Adaptor Proteins
030217 neurology & neurosurgery
Research Article
Zdroj: BMC Neuroscience
BMC Neuroscience, Vol 20, Iss 1, Pp 1-12 (2019)
ISSN: 1471-2202
Popis: Background Mammalian Shc (Src homology and collagen) proteins comprise a family of four phosphotyrosine adaptor molecules which exhibit varied spatiotemporal expression and signaling functions. ShcD is the most recently discovered homologue and it is highly expressed in the developing central nervous system (CNS) and adult brain. Presently however, its localization within specific cell types of mature neural structures has yet to be characterized. Results In the current study, we examine the expression profile of ShcD in the adult rat CNS using immunohistochemistry, and compare with those of the neuronally enriched ShcB and ShcC proteins. ShcD shows relatively widespread distribution in the adult brain and spinal cord, with prominent levels of staining throughout the olfactory bulb, as well as in sub-structures of the cerebellum and hippocampus, including the subgranular zone. Co-localization studies confirm the expression of ShcD in mature neurons and progenitor cells. ShcD immunoreactivity is primarily localized to axons and somata, consistent with the function of ShcD as a cytoplasmic adaptor. Regional differences in expression are observed among neural Shc proteins, with ShcC predominating in the hippocampus, cerebellum, and some fiber tracts. Interestingly, ShcD is uniquely expressed in the olfactory nerve layer and in glomeruli of the main olfactory bulb. Conclusions Together our findings suggest that ShcD may provide a distinct signaling contribution within the olfactory system, and that overlapping expression of ShcD with other Shc proteins may allow compensatory functions in the brain.
Databáze: OpenAIRE
Externí odkaz: