Involvement of Intracellular Ca2+ in the Regulatory Volume Decrease After Hyposmotic Swelling in MDCK Cells
| Autor: | Miku Tamura, Megumi Kaneko, Ryu Nakamura, Naoko Iida-Tanaka, Toru Kawanishi, Iyuki Namekata, Hikaru Tanaka, Koki Shigenobu |
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| Rok vydání: | 2007 |
| Předmět: |
Time Factors
Thapsigargin chemistry.chemical_element Biology Calcium Kidney Cell Line law.invention chemistry.chemical_compound Dogs Confocal microscopy law medicine Animals Cell Size Pharmacology Microscopy Confocal lcsh:RM1-950 Osmolar Concentration Cell biology lcsh:Therapeutics. Pharmacology medicine.anatomical_structure Microscopy Fluorescence chemistry Cytoplasm Cell culture Molecular Medicine Swelling medicine.symptom Intracellular |
| Zdroj: | Journal of Pharmacological Sciences, Vol 104, Iss 4, Pp 397-401 (2007) |
| ISSN: | 1347-8648 1347-8613 |
| Popis: | We examined the source of Ca2+ involved in the volume regulation of Madin-Darby canine kidney (MDCK) cells with confocal microscopy and fluoroprobes. Hyposmosis induced a transient increase in cell volume, as well as cytoplasmic Ca2+, which peaked at 3 to 5 min and gradually decreased to reach the initial value within about 30 min. This late decrease in cell volume, as well as the transient rise in cytoplasmic Ca2+, was reduced in Ca2+-free solution and was abolished by pretreatment with thapsigargin. In conclusion, Ca2+ released from the intracellular store contributes to the regulatory volume decrease following hyposmotic swelling in MDCK cells. Keywords:: hyposmosis, cell volume, intracellular Ca2+ |
| Databáze: | OpenAIRE |
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