Design and synthesis of triazolyl-napthyl derivative of β-amyrin and its in vitro anti-cancer and apoptotic activities in human nasopharyngeal carcinoma (HK-1) cell line
| Autor: | Hong-Zhou Ge, Shi Li, Yong-Gang Xie, Zhen Li |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Human nasopharyngeal carcinoma Cell cycle checkpoint Biology Flow cytometry 03 medical and health sciences 0302 clinical medicine ?-amyrin medicine Viability assay Fragmentation (cell biology) Anti-cancer Apoptotic HK-1 cell line Triazolyl-napthyl β-amyrin Pharmacology medicine.diagnostic_test lcsh:RM1-950 Cell cycle Molecular biology Cell biology 030104 developmental biology lcsh:Therapeutics. Pharmacology Cell culture Apoptosis 030220 oncology & carcinogenesis Cancer cell |
| Zdroj: | Bangladesh Journal of Pharmacology, Vol 11, Iss 1, Pp 168-174 (2016) Bangladesh Journal of Pharmacology, Vol 11, Iss 1 (2016) |
| ISSN: | 1991-0088 |
| Popis: | The purpose of this research work was to design and synthesize triazolyl-napthyl derivative of β-amyrin (TNB) using click chemistry approach. Cytotoxic activity of TNB was evaluated against HK-1 human nasopharyngeal carcinoma cells using MTT cell viability assay. Fluorescence microscopy indicated cellular morphological changes induced by TNB. Flow cytometry was involved to demonstrate effects of this compound on cell cycle and apoptosis. The results revealed that TNB inhibited in vitro cancer cell growth in dose- and time-dependent manner. The IC50 values of TNB at 24 and 48 hours time intervals were found to be 47.2 and 32.5 µM respectively. TNB-treated cells exhibited significant dense staining fragmentation called apoptotic bodies, which inferred an early apoptotic event. DNA ladder was more apparent with the increasing TNB dose. However, no DNA fragments were observed in the control groups. TNB also induced sub-G1 cell cycle arrest and increased fraction of HK-1 apoptotic cells. |
| Databáze: | OpenAIRE |
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