A model for correcting the fluorescence signal from a free-falling depth profiler
| Autor: | C. Moldaenke, Ulf-Peter Hansen, Martin Beutler, Jens Schimanski |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Chlorophyll
Environmental Engineering Chlorophyta Signal Fluorescence chemistry.chemical_compound Optics Algae Phytoplankton Waste Management and Disposal Chlorophyll fluorescence Water Science and Technology Civil and Structural Engineering biology business.industry Ecological Modeling fungi biology.organism_classification Pollution Wavelength Spectrometry Fluorescence chemistry Biological system business |
| Zdroj: | Water research. 40(8) |
| ISSN: | 0043-1354 |
| Popis: | Measuring chlorophyll fluorescence at five different wavelengths provides the discrimination of four phytoplankton groups. Here the problems associated with a free-falling depth profiler for phytoplankton discrimination are considered. When F 0 , F , and F m are determined sequentially in the same measuring cell, then the algae inside the cell have a different light history. It depends on their different locations in the cell as caused by the induction curve of chlorophyll fluorescence. Mathematical algorithms are developed which enable the calculation of the concentrations of individual phytoplankton groups from the integral fluorescence signal (averaged for 1 s) for different velocities of the falling probe. The theory requires the knowledge of the fluorescence behaviour of phytoplankton in stationary suspensions. The predictions of the model are compared with measurements in flowing suspensions containing chlorophyta, cyanobacteria, cryptophyta and diatoms. The comparison shows the reliability of the algorithms. The application of the algorithms is indispensable for dark-adapted cells and is less important for light-adapted cells. |
| Databáze: | OpenAIRE |
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