Functional analysis of CaRAP1 , encoding the Repressor/activator protein 1 of Candida albicans
| Autor: | Klaus-Jörg Rieger, Joachim Morschhäuser, Kajal Biswas |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
endocrine system
Telomere-Binding Proteins Saccharomyces cerevisiae Mutant Repressor Shelterin Complex Fungal Proteins Candida albicans Genetics Gene Fungal protein Genes Essential biology Genetic Complementation Test General Medicine biology.organism_classification Molecular biology Yeast Corpus albicans enzymes and coenzymes (carbohydrates) Phenotype Mutation Cell Division Gene Deletion Transcription Factors |
| Zdroj: | Gene. 307:151-158 |
| ISSN: | 0378-1119 |
| DOI: | 10.1016/s0378-1119(03)00456-6 |
| Popis: | The RAP1 gene (repressor/activator protein 1) encodes a transcription factor and telomere binding protein that is essential for viability in the budding yeast Saccharomyces cerevisiae. The genome sequence of the opportunistic fungal pathogen Candida albicans contains a RAP1 homologue. We generated C. albicans mutants in which both RAP1 alleles were deleted. The Deltarap1 mutants grew as well as the wild-type parental strain and formed normal germ tubes and hyphae in response to a variety of inducing conditions. However, under conditions that promote budding yeast growth in the wild-type strain, the Deltarap1 mutants formed both yeast and pseudohyphal cells. This phenotype was reverted upon reintroduction of a functional RAP1 copy. Our results demonstrate that RAP1 is a non-essential gene in C. albicans which is required to repress the formation of pseudohyphae under conditions favouring growth as budding yeast. |
| Databáze: | OpenAIRE |
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