A cellular automaton model for the proliferation of migrating contact-inhibited cells
| Autor: | Larry V. McIntire, Yih Lee, Stylianos Kouvroukoglou, Kyriacos Zygourakis |
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| Rok vydání: | 1995 |
| Předmět: |
Time Factors
Cell division medicine.medical_treatment Biophysics Motility 02 engineering and technology Biology Pulmonary Artery Models Biological 03 medical and health sciences Automation Random Allocation Cell Movement medicine Animals Cells Cultured 030304 developmental biology 0303 health sciences Cell growth Contact Inhibition Growth factor Cell Cycle Contact inhibition Reproducibility of Results Cell migration Cell cycle 021001 nanoscience & nanotechnology Cellular automaton Cell biology Kinetics Cattle Endothelium Vascular 0210 nano-technology Algorithms Cell Division Research Article |
| Zdroj: | Biophysical journal. 69(4) |
| ISSN: | 0006-3495 |
| Popis: | A cellular automaton is used to develop a model describing the proliferation dynamics of populations of migrating, contact-inhibited cells. Simulations are carried out on two-dimensional networks of computational sites that are finite-state automata. The discrete model incorporates all the essential features of the cell locomotion and division processes, including the complicated dynamic phenomena occurring when cells collide. In addition, model parameters can be evaluated by using data from long-term tracking and analysis of cell locomotion. Simulation results are analyzed to determine how the competing processes of contact inhibition and cell migration affect the proliferation rates. The relation between cell density and contact inhibition is probed by following the temporal evolution of the population-average speed of locomotion. Our results show that the seeding cell density, the population-average speed of locomotion, and the spatial distribution of the seed cells are crucial parameters in determining the temporal evolution of cell proliferation rates. The model successfully predicts the effect of cell motility on the growth of isolated megacolonies of keratinocytes, and simulation results agree very well with experimental data. Model predictions also agree well with experimentally measured proliferation rates of bovine pulmonary artery endothelial cells (BPAE) cultured in the presence of a growth factor (bFGF) that up-regulates cell motility. |
| Databáze: | OpenAIRE |
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