Nanostructured Assemblies for Dental Application

Autor: Youssef Haïkel, Youri Arntz, Sophie C. Gangloff, Fabien Garnier, Denise Al Alam, Nadia Benkirane-Jessel, Carlos Mendoza-Palomares, Ludovic Richert, Marie Helms, Florence Fioretti, Simon Rinckenbach
Přispěvatelé: Biomatériaux et ingénierie tissulaire, Université Louis Pasteur - Strasbourg I-Institut National de la Santé et de la Recherche Médicale (INSERM), Faculté de Chirurgie Dentaire, Université de Reims Champagne-Ardenne (URCA), Laboratoire de Biophotonique et Pharmacologie - UMR 7213 (LBP), Réseau nanophotonique et optique, Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Physiopathologie, Pharmacologie et Ingénierie articulaires (PPIA), Université Henri Poincaré - Nancy 1 (UHP)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Réseau nanophotonique et optique, Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA)), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)
Jazyk: angličtina
Rok vydání: 2010
Předmět:
endocrine system
Materials science
Lipopolysaccharide
Root canal
pulp fibroblasts
General Physics and Astronomy
Inflammation
02 engineering and technology
03 medical and health sciences
chemistry.chemical_compound
Nanocapsules
multilayered films
Cell Adhesion
medicine
General Materials Science
Secretion
melanocortin
Fibroblast
[SDV.IB.BIO]Life Sciences [q-bio]/Bioengineering/Biomaterials
Cells
Cultured
Dental Pulp
Cell Proliferation
030304 developmental biology
0303 health sciences
integumentary system
Cell growth
[CHIM.ORGA]Chemical Sciences/Organic chemistry
General Engineering
Biomaterial
Fibroblasts
021001 nanoscience & nanotechnology
cytokines
3. Good health
Cell biology
medicine.anatomical_structure
nanostructured assemblies
[CHIM.POLY]Chemical Sciences/Polymers
Polyglutamic Acid
chemistry
alpha-MSH
inflammation
Immunology
Pulp (tooth)
medicine.symptom
0210 nano-technology
Dendri Graft poly-L-lysines
hormones
hormone substitutes
and hormone antagonists
Zdroj: ACS Nano
ACS Nano, American Chemical Society, 2010, 4 (6), pp.3277-3287. ⟨10.1021/nn100713m⟩
ISSN: 1936-0851
Popis: Millions of teeth are saved each year by root canal therapy. Although current treatment modalities offer high levels of success for many conditions, an ideal form of therapy might consist of regenerative approaches in which diseased or necrotic pulp tissues are removed and replaced with healthy pulp tissue to revitalize teeth. Melanocortin peptides (alpha-MSH) possess anti-inflammatory properties in many acute and chronic inflammatory models. Our recent studies have shown that alpha-MSH covalently coupled to poly-l-glutamic acid (PGA-alpha-MSH) retains anti-inflammatory properties on rat monocytes. This study aimed to define the effects of PGA-alpha-MSH on dental pulp fibroblasts. Lipopolysaccharide (LPS)-stimulated fibroblasts incubated with PGA-alpha-MSH showed an early time-dependent inhibition of TNF-alpha, a late induction of IL-10, and no effect on IL-8 secretion. However, in the absence of LPS, PGA-alpha-MSH induced IL-8 secretion and proliferation of pulp fibroblasts, whereas free alpha-MSH inhibited this proliferation. Thus, PGA-alpha-MSH has potential effects in promoting human pulp fibroblast adhesion and cell proliferation. It can also reduce the inflammatory state of LPS-stimulated pulp fibroblasts observed in gram-negative bacterial infections. These effects suggest a novel use of PGA-alpha-MSH as an anti-inflammatory agent in the treatment of endodontic lesions. To better understand these results, we have also used the multilayered polyelectrolyte films as a reservoir for PGA-alpha-MSH by using not only PLL (poly-l-lysine) but also the Dendri Graft poly-l-lysines (DGL(G4)) to be able to adsorb more PGA-alpha-MSH. Our results indicated clearly that, by using PGA-alpha-MSH, we increase not only the viability of cells but also the proliferation. We have also analyzed at the nanoscale by atomic force microscopy these nanostructured architectures and shown an increase of thickness and roughness in the presence of PGA-alpha-MSH incorporated into the multilayered film (PLL-PGA-alpha-MSH)(10) or (DGL(G4)-PGA-alpha-MSH)(10) in accordance with the increase of the proliferation of the cells growing on the surface of these architectures. We report here the first use of nanostructured and functionalized multilayered films containing alpha-MSH as a new active biomaterial for endodontic regeneration.
Databáze: OpenAIRE
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