In vitroinhibition of human liver cytochrome P450 (CYP) and UDP-glucuronosyltransferase (UGT) enzymes by rose bengal: system-dependent effects on inhibitory potential

Autor:	David B. Buckley, E. A. Wachter, Jamie Singer, Jennifer R. Horkman, Faraz Kazmi, Brian D. Smith, Lois J. Haupt
Rok vydání:	2014
Předmět:	Male UGT1A6 Light Cell Survival Health Toxicology and Mutagenesis Drug Evaluation Preclinical Pharmacology Toxicology Biochemistry Inhibitory Concentration 50 chemistry.chemical_compound Cytochrome P-450 Enzyme System Rose bengal Cytochrome P-450 CYP3A Cytochrome P-450 Enzyme Inhibitors Humans Drug Interactions Glucuronosyltransferase IC50 Cells Cultured Cryopreservation chemistry.chemical_classification Rose Bengal CYP3A4 biology CYP1A2 Cytochrome P450 General Medicine Enzyme chemistry Hepatocytes Microsomes Liver biology.protein Microsome Female
Zdroj:	Xenobiotica. 44:606-614
ISSN:	1366-5928 0049-8254
Popis:	1. Rose bengal (4,5,6,7-tetrachloro-2',4',5',7'-tetraiodofluorescein) is being developed for the treatment of cutaneous melanoma and hepatocellular carcinoma. Interestingly, rose bengal can generate singlet oxygen species upon exposure to light. 2. We evaluated rose bengal as an in vitro inhibitor of cytochrome P450 (CYP) or UDP-glucuronosyltransferase (UGT) enzymes in both human liver microsomes (HLM) and cryopreserved human hepatocytes (CHHs) under both yellow light and dark conditions. 3. Rose bengal directly inhibited CYP3A4/5 and UGT1A6 in HLM under yellow light with inhibitor concentration that causes 50% inhibition (IC50) values of 0.072 and 0.035 μM, respectively; whereas much less inhibition was observed in the dark with the IC50 values increasing 43- and 120-fold, respectively. To determine if a more physiologically-relevant test system could be protected from such an effect, rose bengal was evaluated as an inhibitor of CYP1A2, 2B6, 2C8, 2C9, 2C19, 2D6, 3A4/5 and UGT enzymes in CHH. All IC50 values were similar (64 ± 8 μM) and little to no effect of light on inhibitory potential was observed. 4. Given the IC50 values in CHH increased an order of magnitude compared to HLM and the atypical pharmacokinetics of the drug, the risk of rose bengal to cause clinically relevant drug-drug interactions is likely low, particularly when administered to cancer patients on an intermittent schedule.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f4c121d2be2ffd1df9318d5de0267f99 https://doi.org/10.3109/00498254.2013.878814 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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