T cell subsets and sIL-2R/IL-2 levels in patients with glaucoma
Autor: | Hao Yu, Rajkumar V. Patil, Mae O. Gordon, Junjie Yang, Martin B. Wax |
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Rok vydání: | 2001 |
Předmět: |
CD4-Positive T-Lymphocytes
Male Interleukin 2 Cellular immunity genetic structures medicine.medical_treatment T cell CD4-CD8 Ratio Enzyme-Linked Immunosorbent Assay Lymphocyte proliferation CD8-Positive T-Lymphocytes Lymphocyte Activation Antigen Antigens CD T-Lymphocyte Subsets medicine Humans Phytohemagglutinins Aged Immunity Cellular business.industry Receptors Interleukin-2 HLA-DR Antigens T lymphocyte Flow Cytometry eye diseases Ophthalmology Cytokine medicine.anatomical_structure Immunology Interleukin-2 Female business Glaucoma Open-Angle CD8 medicine.drug |
Zdroj: | American Journal of Ophthalmology. 131:421-426 |
ISSN: | 0002-9394 |
Popis: | PURPOSE: We hypothesize that cellular immunity may have a previously unrecognized role in glaucomatous optic neuropathy. The purpose of this study is to analyze subsets of T cells and the levels of cytokine IL-2 and the soluble IL-2 receptor in peripheral blood from patients with normal pressure glaucoma (NPG) or primary open angle glaucoma (POAG) in comparison to age-matched control subjects. METHODS: In this study, 38 patients (20 NPG; 18 POAG) and 19 controls were included. sIL-2R and IL-2 were assayed by ELISA. T cell subsets were analyzed by flow cytometry and lymphocyte proliferation was used to measure the reactive ability of T cells to phytohemagglutinin (PHA). RESULTS: The frequency of CD8 + HLA-DR + lymphocytes were increased in patients with NPG ( P = 0.008), and CD3 + CD8 + lymphocytes increased in both NPG ( P = 0.03) and POAG patients ( P = 0.0004). CD5 + lymphocytes were higher only in POAG patients ( P = 0.0012). In comparison to controls, the ratio of CD4 + /CD8 + lymphocytes was similar in both groups. The mean concentrations of sIL-2R in NPG ( P = 0.011) and POAG ( P = 0.0023) patients were higher than that found in control subjects although IL-2 concentrations were similar in these groups. In addition, the reactive ability of T lymphocytes to the non-specific reagent (PHA) was reduced significantly in NPG ( P = 0.02) and POAG patients ( P =0.04). CONCLUSION: The alterations of the cellular immune system in patients with glaucoma support our hypothesis that the immune system may play an important role in the initiation and/or sustainment of glaucomatous optic neuropathy in some patients. |
Databáze: | OpenAIRE |
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