Anti-hyperuricemic and nephroprotective effects of Modified Simiao Decoction in hyperuricemic mice
| Autor: | Xiao Yuan, Ping Huang, Chen-Huan Yu, Chong-Mei Zhu, Jian Hua |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Male
Xanthine Oxidase Uricosuric Organic Anion Transporters Allopurinol Hyperuricemia Pharmacology Kidney Protective Agents Excretion Mice chemistry.chemical_compound Organic Anion Transport Protein 1 Malondialdehyde Drug Discovery medicine Animals Mice Inbred ICR Creatinine Traditional medicine Superoxide Dismutase Kidney metabolism Uricosuric Agents medicine.disease Oxonic Acid medicine.anatomical_structure Liver chemistry Uric acid Drugs Chinese Herbal Phytotherapy medicine.drug |
| Zdroj: | Journal of Ethnopharmacology. 142:248-252 |
| ISSN: | 0378-8741 |
| DOI: | 10.1016/j.jep.2012.04.052 |
| Popis: | Ethnopharmacological relevance Modified Simiao Decoction (MSD), based on clinical experience, has been used for decades and famous for its efficiency in treating hyperuricemic and gouty diseases. Aim of the study To investigate the effects of MSD on anti-hyperuricemic and nephroprotective effects are involved in potassium oxonate-induced hyperuricemic mice. Materials and methods The effects of MSD were investigated in hyperuricemic mice induced by potassium oxonate. MSD were fed to hyperuricemic mice daily at a dose of 0.45, 0.90, 1.80 g/kg for 10 days, and allopurinol (5 mg/kg) was given as a positive control. Serum and urine levels of uric acid and creatinine, and fractional excretion of uric acid (FEUA) were determined by colorimetric method. Its nephroprotective effects were evaluated by determining a panel of oxidative stress markers after the intervention in hyperuricemic mice. Simultaneously, protein levels of urate transporter 1 (URAT1) and organic anion transporter 1 (OAT1) in the kidney were analyzed by Western blotting. Results MSD could inhibit XOD activities in serum and liver, decrease levels of serum uric acid, serum creatinine and BUN, and increased levels of urine uric acid, urine creatinine, FEUA dose-dependently through down-regulation of URAT1 and up-regulation of OAT1 protein expressions in the renal tissue of hyperuricemic mice. It also effectively reversed oxonate-induced alterations on renal MDA levels and SOD activities in this model. Conclusion MSD processes uricosuric and nephroprotective actions by regulating renal urate transporters and enhancing antioxidant enzymes activities to improve renal dysfunction in hyperuricemic mice. |
| Databáze: | OpenAIRE |
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