Removal of the Bloom Syndrome DNA Helicase Extends the Utility of Imprecise Transposon Excision for Making Null Mutations in Drosophila
| Autor: | Daniel P. Kane, Alice Witsell, Sarah Rubin, Mitch McVey |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Transposable element
Male DNA Repair DNA repair Genome Insect medicine.disease_cause Notes Genetics medicine Animals Drosophila Proteins Bloom syndrome DNA Breaks Double-Stranded Gene Mutation biology fungi DNA Helicases Helicase medicine.disease biology.organism_classification Mutagenesis Insertional Drosophila melanogaster Fertility Bloom syndrome protein biology.protein DNA Transposable Elements Female Gene Deletion |
| Popis: | Transposable elements are frequently used in Drosophila melanogaster for imprecise excision screens to delete genes of interest. However, these screens are highly variable in the number and size of deletions that are recovered. Here, we show that conducting excision screens in mus309 mutant flies that lack DmBlm, the Drosophila ortholog of the Bloom syndrome protein, increases the percentage and overall size of flanking deletions recovered after excision of either P or Minos elements. |
| Databáze: | OpenAIRE |
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